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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RD3 Polyclonal antibody

RD3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14855-1-AP

Synonyms

C1orf36, LCA12, Protein RD3, RD3, retinal degeneration 3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RD3 Polyclonal antibody (14855-1-AP)

    WB analysis using 14855-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14855-1-AP (RD3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of rat eye tissue using RD3 Polyclonal antibody (14855-1-AP)

    IHC staining of rat eye using 14855-1-AP

    Immunohistochemical analysis of paraffin-embedded rat eye tissue slide using 14855-1-AP (RD3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat eye tissue using RD3 Polyclonal antibody (14855-1-AP)

    IHC staining of rat eye using 14855-1-AP

    Immunohistochemical analysis of paraffin-embedded rat eye tissue slide using 14855-1-AP (RD3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inmouse retina tissue, rat retina tissue
Positive IHC detected inrat eye tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14855-1-AP targets RD3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RD3 fusion protein Ag6641 相同性解析による交差性が予測される生物種
Full Name retinal degeneration 3
Calculated molecular weight22.7 kDa
Observed molecular weight 23 kDa
GenBank accession numberBC065541
Gene symbol RD3
Gene ID (NCBI) 343035
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RD3, also named as C1orf36, is a part of subnuclear protein complexes involved in diverse processes, such as transcription and splicing. It is associated with Retinal Degeneration.

Protocols

Product Specific Protocols
WB protocol for RD3 antibody 14855-1-APDownload protocol
IHC protocol for RD3 antibody 14855-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols