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NELFE Polyclonal antibody

Name: NELFE Polyclonal antibody
Brand: Proteintech
SKU: 10705-1-AP
Rating: 5.00 (1 reviews)

NELFE Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ChIP, RIP, ELISA

Conjugate

Unconjugated

Publications

Reviews (1)

Cat no : 10705-1-AP

Synonyms

RNA-binding protein RD, RDBP, RD, NELF-E, NELF E

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICCFC (Intra)

WB analysis of HEK-293 using 10705-1-AP
WB result of NELFE antibody (10705-1-AP; 1:60000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NELFE transfected HEK-293 cells.
WB analysis using 10705-1-AP
Various lyastes were subjected to SDS PAGE followed by western blot with 10705-1-AP (NELFE antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
WB analysis using 10705-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10705-1-AP (NELFE antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis using 10705-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10705-1-AP (NELFE antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
WB analysis of mouse eye using 10705-1-AP
mouse eye tissue were subjected to SDS PAGE followed by western blot with 10705-1-AP (NELFE antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of mouse placenta using 10705-1-AP
mouse placenta tissue were subjected to SDS PAGE followed by western blot with 10705-1-AP (NELFE antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
IP experiment of mouse brain using 10705-1-AP
IP result of anti-NELFE (IP:10705-1-AP, 4ug; Detection:10705-1-AP 1:6000) with mouse brain tissue lysate 1840 ug.
IHC staining of human prostate cancer using 10705-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10705-1-AP (NELFE antibody) at dilution of 1:100 (under 40x lens).
IF Staining of HeLa using 10705-1-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NELFE antibody (10705-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).
FC experiment of hela using 10705-1-AP
1X10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human NELFE (10705-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).
×
1X10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human NELFE (10705-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Cat No. 10705-1-AP

Size/Concentration

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HEK-293 cells, HeLa cells, mouse brain tissue, mouse eye tissue, mouse placenta tissue, mouse kidney tissue, rat kidney tissue, rat brain tissue
Positive IP detected in	mouse brain tissue
Positive IHC detected in	human prostate cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells
Positive FC (Intra) detected in	hela cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:12000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:200
Immunofluorescence (IF)/ICC	IF/ICC : 1:300-1:1200
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 2 publications below
WB	See 16 publications below
IHC	See 4 publications below
IF	See 2 publications below
ChIP	See 4 publications below
RIP	See 1 publications below

Product Information

10705-1-AP targets NELFE in WB, IHC, IF/ICC, FC (Intra), IP, ChIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	NELFE fusion protein Ag1186 相同性解析による交差性が予測される生物種
Full Name	RD RNA binding protein
Calculated molecular weight	37 kDa, 43 kDa
Observed molecular weight	43 kDa
GenBank accession number	BC011600
Gene symbol	NELFE
Gene ID (NCBI)	7936
RRID	AB_513966
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

RDBP, also named NELF-E and RD, belongs to the RRM NELF-E family. It is an essential component of the NELF complex which is a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, a complex acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex. RDBP was recently found implicated in RNA polymerase II pausing by association with Hsp90. Catalog# 10705-1-AP is a widely used rabbit polyclonal antibody obtained by immunizing animals with full-length RDBP of human origin.

Protocols

Product Specific Protocols
WB protocol for NELFE antibody 10705-1-AP	Download protocol
IHC protocol for NELFE antibody 10705-1-AP	Download protocol
IF protocol for NELFE antibody 10705-1-AP	Download protocol
IP protocol for NELFE antibody 10705-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	IF	Nature The landscape of RNA Pol II binding reveals a stepwise transition during ZGA. Authors - Bofeng Liu View Article
human, mouse	WB	Cell Hsp90 globally targets paused RNA polymerase to regulate gene expression in response to environmental stimuli. Authors - Sawarkar Ritwick R View Article
mouse	WB,chIP	Nat Commun Negative elongation factor complex enables macrophage inflammatory responses by controlling anti-inflammatory gene expression. Authors - Li Yu View Article
mouse	WB	Nat Commun RNA polymerase II pausing factor NELF in CD8+ T cells promotes antitumor immunity. Authors - Bogang Wu View Article
mouse	WB	Dev Cell Negative elongation factor regulates muscle progenitor expansion for efficient myofiber repair and stem cell pool repopulation. Authors - Daniel C L Robinson View Article
mouse	WB,IHC	Proc Natl Acad Sci U S A Immediate mediators of the inflammatory response are poised for gene activation through RNA polymerase II stalling. Authors - Adelman Karen K View Article

Reviews

Add a review

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

HUAI-CHIN (Verified Customer) (02-14-2019)

This antibody works great in Western. Gives me clean single band.

Applications: Western Blot,
Primary Antibody Dilution: 1:1000
Cell Tissue Type: Mouse embryonic fibroblast

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NELFE Polyclonal antibody

NELFE Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Validation Data Gallery

WB analysis of HEK-293 using 10705-1-AP

WB analysis using 10705-1-AP

WB analysis using 10705-1-AP

WB analysis using 10705-1-AP

WB analysis of mouse eye using 10705-1-AP

WB analysis of mouse placenta using 10705-1-AP

IP experiment of mouse brain using 10705-1-AP

IHC staining of human prostate cancer using 10705-1-AP

IF Staining of HeLa using 10705-1-AP

FC experiment of hela using 10705-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

The landscape of RNA Pol II binding reveals a stepwise transition during ZGA.

Hsp90 globally targets paused RNA polymerase to regulate gene expression in response to environmental stimuli.

Negative elongation factor complex enables macrophage inflammatory responses by controlling anti-inflammatory gene expression.

RNA polymerase II pausing factor NELF in CD8+ T cells promotes antitumor immunity.

Negative elongation factor regulates muscle progenitor expansion for efficient myofiber repair and stem cell pool repopulation.

Immediate mediators of the inflammatory response are poised for gene activation through RNA polymerase II stalling.

Reviews

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