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RDH10 Polyclonal antibody

RDH10 Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 14644-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, IP, ELISA

RDH10, Retinol dehydrogenase 10, SDR16C4

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -


ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive WB detected inHepG2 cells, A549 cells, mouse liver tissue, mouse kidney tissue, rat kidney tissue
Positive IP detected inmouse liver tissue
Positive IHC detected inmouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14644-1-AP targets RDH10 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, insect
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RDH10 fusion protein Ag6237 相同性解析による交差性が予測される生物種
Full Name retinol dehydrogenase 10 (all-trans)
Calculated molecular weight 38 kDa
Observed molecular weight 39 kDa
GenBank accession numberBC067131
Gene Symbol RDH10
Gene ID (NCBI) 157506
RRIDAB_2177866
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8IZV5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RDH10(Retinol dehydrogenase 10) is a strictly NAD+-dependent enzyme with multisubstrate specificity that recognizes cis-retinols as well as all-trans-retinol as substrates. RDH10-mediated oxidation of retinol plays as important a role in the control and regulation of RA production during embryogenesis as does the subsequent RALDH-mediated reaction(PMID: 22319578).

Protocols

Product Specific Protocols
WB protocol for RDH10 antibody 14644-1-APDownload protocol
IHC protocol for RDH10 antibody 14644-1-APDownload protocol
IP protocol for RDH10 antibody 14644-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Nat Commun

Generation of a ciliary margin-like stem cell niche from self-organizing human retinal tissue.

Authors - Atsushi Kuwahara
mouseWB

Proc Natl Acad Sci U S A

Retinoic acid signaling determines the fate of uterine stroma in the mouse Müllerian duct.

Authors - Tadaaki Nakajima
mouse

Curr Biol

Multiple Isoforms of Nesprin1 Are Integral Components of Ciliary Rootlets.

Authors - Chloe Potter
mouseWB

Cell Prolif

Omega-3 polyunsaturated fatty acids and its metabolite 12-HEPE rescue busulfan disrupted spermatogenesis via target to GPR120

Authors - Jun Jing
mouseIF

Cell Rep

Macrophages Contribute to the Spermatogonial Niche in the Adult Testis.

Authors - Tony DeFalco
humanIF

Stem Cell Reports

Intercellular Adhesion-Dependent Cell Survival and ROCK-Regulated Actomyosin-Driven Forces Mediate Self-Formation of a Retinal Organoid.

Authors - Albert Lowe
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