Validation Data Gallery
Tested Applications
| Positive IHC detected in | human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HaCaT cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
11077-1-AP targets RDH11 in IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | RDH11 fusion protein Ag1485 相同性解析による交差性が予測される生物種 |
| Full Name | retinol dehydrogenase 11 (all-trans/9-cis/11-cis) |
| Calculated molecular weight | 35 kDa |
| Observed molecular weight | 35 kDa |
| GenBank accession number | BC011727 |
| Gene Symbol | RDH11 |
| Gene ID (NCBI) | 51109 |
| RRID | AB_2177870 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8TC12 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
RDH11(Retinol dehydrogenase 11) is also named as ARSDR1(androgen-regulated short-chain dehydrogenase/reductase 1 ), PSDR1(prostate short-chain dehydrogenase/reductase 1) and belongs to the short-chain dehydrogenases/reductases (SDR) family. RDH11 is involved in steroid synthesis and/or degradation in normal and neoplastic prostate epithelium, and, as such, it may be a key enzyme involved in maintaining intracellular balance of steroid hormones in these cells(PMID:11245473). The deduced protein contains 318 amino acids, and the transcript contains 2 potential polyadenylation signals. It has 2 isoforms produced by alternative splicing.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for RDH11 antibody 11077-1-AP | Download protocol |
| IF protocol for RDH11 antibody 11077-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |