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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RDH12 Polyclonal antibody

RDH12 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13289-3-AP

Synonyms

LCA13, LCA3, RDH12, Retinol dehydrogenase 12, SDR7C2

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using RDH12 Polyclonal antibody (13289-3-AP)

    WB analysis using 13289-3-AP

    mouse eye tissue were subjected to SDS PAGE followed by western blot with 13289-3-AP (RDH12 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse eye tissue using RDH12 Polyclonal antibody (13289-3-AP)

    IHC staining of mouse eye using 13289-3-AP

    Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 13289-3-AP (RDH12 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse eye tissue using RDH12 Polyclonal antibody (13289-3-AP)

    IHC staining of mouse eye using 13289-3-AP

    Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 13289-3-AP (RDH12 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inmouse eye tissue
Positive IHC detected inmouse eye tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13289-3-AP targets RDH12 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RDH12 fusion protein Ag3850 相同性解析による交差性が予測される生物種
Full Name retinol dehydrogenase 12 (all-trans/9-cis/11-cis)
Calculated molecular weight 316 aa, 35 kDa
Observed molecular weight 35 kDa
GenBank accession numberBC025724
Gene symbol RDH12
Gene ID (NCBI) 145226
RRIDAB_2178131
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for RDH12 antibody 13289-3-APDownload protocol
IHC protocol for RDH12 antibody 13289-3-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols