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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RDH14 Polyclonal antibody

RDH14 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16283-1-AP

Synonyms

Short chain dehydrogenase/reductase family 7C member 4, SDR7C4, Retinol dehydrogenase 14, PAN2, EC:1.1.1.300

Validation Data Gallery

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  • Western Blot (WB) analysis of human heart tissue using RDH14 Polyclonal antibody (16283-1-AP)

    WB analysis of human heart using 16283-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 16283-1-AP (RDH14 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using RDH14 Polyclonal antibody (16283-1-AP)

    WB analysis of A549 using 16283-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 16283-1-AP (RDH14 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human kidney tissue using RDH14 Polyclonal antibody (16283-1-AP)

    IHC staining of human kidney using 16283-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16283-1-AP (RDH14 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using RDH14 Polyclonal antibody (16283-1-AP)

    IHC staining of human kidney using 16283-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16283-1-AP (RDH14 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inhuman heart tissue, A549 cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16283-1-AP targets RDH14 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RDH14 fusion protein Ag9386 相同性解析による交差性が予測される生物種
Full Name retinol dehydrogenase 14 (all-trans/9-cis/11-cis)
Calculated molecular weight 336 aa, 37 kDa
Observed molecular weight 37 kDa
GenBank accession numberBC009830
Gene symbol RDH14
Gene ID (NCBI) 57665
RRIDAB_2158906
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for RDH14 antibody 16283-1-APDownload protocol
IHC protocol for RDH14 antibody 16283-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols