Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse eye tissue, rat eye tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
13140-1-AP targets RDH5 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | RDH5 fusion protein Ag3784 相同性解析による交差性が予測される生物種 |
| Full Name | retinol dehydrogenase 5 (11-cis/9-cis) |
| Calculated molecular weight | 318 aa, 35 kDa |
| Observed molecular weight | 32 kDa |
| GenBank accession number | BC028298 |
| Gene Symbol | RDH5 |
| Gene ID (NCBI) | 5959 |
| RRID | AB_2918031 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q92781 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
RDH5, also named as RDH1, SDR9C5 and HSD17B9, belongs to short-chain dehydrogenases/reductases (SDR) family. RDH5 catalyzes the oxidation of cis-isomers of retinol in an NAD-dependent manner for the final step in the biosynthesis of 11-cis retinaldehyde. Mutations in this gene are associated with fundus albipunctatus, an autosomal recessive eye disease characterized by stationary night blindness and accumulation of white spots in the retina (PubMed:11675386). The predicted size of this protein is 35 kDa. Some paper have reported that the molecular weight is 32 kDa and could form a dimer of about 60 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for RDH5 antibody 13140-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |