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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RDM1 Monoclonal antibody

RDM1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1C10B4

Cat no : 66941-1-Ig

Synonyms

RAD52 homolog B, RAD52 motif 1, RAD52B, RDM1

Validation Data Gallery

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  • Western Blot (WB) analysis of PC-3 cells using RDM1 Monoclonal antibody (66941-1-Ig)

    WB analysis of PC-3 using 66941-1-Ig

    PC-3 cells were subjected to SDS PAGE followed by western blot with 66941-1-Ig (RDM1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HSC-T6 cells using RDM1 Monoclonal antibody (66941-1-Ig)

    WB analysis of HSC-T6 using 66941-1-Ig

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66941-1-Ig (RDM1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of RAW 264.7 cells using RDM1 Monoclonal antibody (66941-1-Ig)

    WB analysis of RAW 264.7 using 66941-1-Ig

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66941-1-Ig (RDM1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using RDM1 Monoclonal antibody (66941-1-Ig)

    WB analysis of HEK-293 using 66941-1-Ig

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66941-1-Ig (RDM1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human thyroid cancer tissue using RDM1 Monoclonal antibody (66941-1-Ig)

    IHC staining of human thyroid cancer using 66941-1-Ig

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 66941-1-Ig (RDM1 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human thyroid cancer tissue using RDM1 Monoclonal antibody (66941-1-Ig)

    IHC staining of human thyroid cancer using 66941-1-Ig

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 66941-1-Ig (RDM1 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human thyroid cancer tissue using RDM1 Monoclonal antibody (66941-1-Ig)

    IHC staining of human thyroid cancer using 66941-1-Ig

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 66941-1-Ig (RDM1 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human thyroid cancer tissue using RDM1 Monoclonal antibody (66941-1-Ig)

    IHC staining of human thyroid cancer using 66941-1-Ig

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 66941-1-Ig (RDM1 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using RDM1 Monoclonal antibody (66941-1-Ig)

    IF Staining of A549 using 66941-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using RDM1 antibody (66941-1-Ig, Clone: 1C10B4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

Positive WB detected inPC-3 cells, HEK-293 cells, HSC-T6 cells, RAW 264.7 cells
Positive IHC detected inhuman thyroid cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:150-1:600
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66941-1-Ig targets RDM1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen RDM1 fusion protein Ag28686 相同性解析による交差性が予測される生物種
Full Name RAD52 motif 1
Calculated molecular weight 284 aa, 32 kDa
Observed molecular weight 32 kDa
GenBank accession numberBC038301
Gene symbol RDM1
Gene ID (NCBI) 201299
RRIDAB_2882265
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RDM1, also named as RAD52B, encodes a RNA recognition motif (RRM)-containing protein confered resistance to the antitumor agent cisplatin. The expression of RDM1 is closely related to the cell proliferation and apoptosis of thyroid carcinoma cells and RDM1 may be a potential marker of lung adenocarcinoma. RDM1 has many isoforms (8 kDa, 12-19 kDa and 25-32 kDa) with long or short N-terminus and a great diversity in the exonic composition of C-terminus generated by alternative pre-mRNA splicing and differential usage of two translational start sites. RDM1 protein can be expressed differently in cytoplasm or nuclear/nucleolar in the absence of stress, proteotoxic stress and mild heat shock, suggesting functional differences among the RDM1 proteins. ( PMID: 28939762, 17905820, 28939762, 30069034)

Protocols

Product Specific Protocols
WB protocol for RDM1 antibody 66941-1-IgDownload protocol
IHC protocol for RDM1 antibody 66941-1-IgDownload protocol
IF protocol for RDM1 antibody 66941-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols