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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RDX Polyclonal antibody

RDX Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, monkey

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 26105-1-AP

Synonyms

DFNB24, radixin, RDX

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RDX Polyclonal antibody (26105-1-AP)

    WB analysis using 26105-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26105-1-AP (RDX antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using RDX Polyclonal antibody (26105-1-AP)

    WB analysis of mouse liver using 26105-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 26105-1-AP (RDX Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using RDX Polyclonal antibody (26105-1-AP)

    WB analysis of HeLa using 26105-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 26105-1-AP (RDX Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using RDX Polyclonal antibody (26105-1-AP)

    WB analysis of Jurkat using 26105-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 26105-1-AP (RDX Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using RDX Polyclonal antibody (26105-1-AP)

    IF Staining of HeLa using 26105-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 26105-1-AP (RDX antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inCOS-7 cells, HeLa cells, Jurkat cells, mouse liver tissue, C6 cells, mouse brain tissue, mouse lung tissue, rat brain tissue
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

26105-1-AP targets RDX in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, monkey samples.

Tested Reactivity human, mouse, rat, monkey
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RDX fusion protein Ag23532 相同性解析による交差性が予測される生物種
Full Name radixin
Calculated molecular weight 583 aa, 68 kDa
Observed molecular weight 70-75 kDa
GenBank accession numberBC047109
Gene symbol RDX
Gene ID (NCBI) 5962
RRIDAB_2880380
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for RDX antibody 26105-1-APDownload protocol
IF protocol for RDX antibody 26105-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols