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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RECQL5 Polyclonal antibody

RECQL5 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 12468-2-AP

Synonyms

EC:5.6.2.4, DNA helicase, RecQ-like type 5, DNA helicase, RecQ like type 5, DNA 3'-5' helicase RecQ5, ATP-dependent DNA helicase Q5

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using RECQL5 Polyclonal antibody (12468-2-AP)

    WB analysis using 12468-2-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12468-2-AP (RECQL5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse testis tissue using RECQL5 Polyclonal antibody (12468-2-AP)

    WB analysis of mouse testis using 12468-2-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 12468-2-AP (RECQL5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using RECQL5 Polyclonal antibody (12468-2-AP)

    IHC staining of human pancreas cancer using 12468-2-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 12468-2-AP (RECQL5 antibody) at dilution of 1:50 (under 10x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using RECQL5 Polyclonal antibody (12468-2-AP)

    IF Staining of HepG2 using 12468-2-AP

    Immunofluorescent analysis of HepG2 cells, using RECQL5 antibody 12468-2-AP at 1:100 dilution and FITC-labeled donkey anti-rabbit IgG(green). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Tested Applications

Positive WB detected inHEK-293T cells, mouse testis tissue
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12468-2-AP targets RECQL5 in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RECQL5 fusion protein Ag3145 相同性解析による交差性が予測される生物種
Full Name RecQ protein-like 5
Calculated molecular weight 435 aa, 49 kDa, 409 kDa
Observed molecular weight 48 kDa, 130-140 kDa
GenBank accession numberBC016911
Gene symbol RECQL5
Gene ID (NCBI) 9400
RRIDAB_2300899
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for RECQL5 antibody 12468-2-APDownload protocol
IHC protocol for RECQL5 antibody 12468-2-APDownload protocol
IF protocol for RECQL5 antibody 12468-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,CoIP

bioRxiv

Senataxin and DNA-PKcs Redundantly Promote Non-Homologous End Joining Repair of DNA Double Strand Breaks During V(D)J Recombination

Authors - Bo-Ruei Chen
View Article