- Featured Product
- KD/KO Validated
REEP1 Polyclonal antibody
REEP1 Polyclonal Antibody for WB, IP, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF, IP, ELISA
Conjugate
Unconjugated
Cat no : 17988-1-AP
Synonyms
Validation Data Gallery
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Tested Applications
| Positive WB detected in | rat brain tissue, mouse brain tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 9 publications below |
| IF | See 1 publications below |
| IP | See 2 publications below |
Product Information
17988-1-AP targets REEP1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | REEP1 fusion protein Ag12418 相同性解析による交差性が予測される生物種 |
| Full Name | receptor accessory protein 1 |
| Calculated molecular weight | 201 aa, 22 kDa |
| Observed molecular weight | 22 kDa |
| GenBank accession number | BC064846 |
| Gene symbol | REEP1 |
| Gene ID (NCBI) | 65055 |
| RRID | AB_2178441 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for REEP1 antibody 17988-1-AP | Download protocol |
| IHC protocol for REEP1 antibody 17988-1-AP | Download protocol |
| IP protocol for REEP1 antibody 17988-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Struct Mol Biol Aurora kinase A-mediated phosphorylation triggers structural alteration of Rab1A to enhance ER complexity during mitosis | ||
Cell Res DNA damage triggers tubular endoplasmic reticulum extension to promote apoptosis by facilitating ER-mitochondria signaling. | ||
Mol Psychiatry Dysfunctional tubular endoplasmic reticulum constitutes a pathological feature of Alzheimer's disease. | ||
Autophagy DIPK2A promotes STX17- and VAMP7-mediated autophagosome-lysosome fusion by binding to VAMP7B. | ||
Hum Mol Genet Reep1 null mice reveal a converging role for hereditary spastic paraplegia proteins in lipid droplet regulation.
| ||
Neurobiol Dis Disease-causing mutated ATLASTIN 3 is excluded from distal axons and reduces axonal autophagy. |
