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  • KD/KO Validated

REEP2 Polyclonal antibody

REEP2 Polyclonal Antibody for WB, IHC, IF-P, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF-P, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Cat no : 15684-1-AP

Synonyms

SGC32445, Receptor expression-enhancing protein 2, C5orf19


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Tested Applications

Positive WB detected inmouse brain tissue, human heart tissue, mouse eye tissue, human skeletal muscle tissue, rat brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse brain tissue
Positive FC (Intra) detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15684-1-AP targets REEP2 in WB, IHC, IF-P, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, monkey
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen REEP2 fusion protein Ag8186 相同性解析による交差性が予測される生物種
Full Name receptor accessory protein 2
Calculated molecular weight 252 aa, 28 kDa
Observed molecular weight 28 kDa
GenBank accession numberBC006218
Gene symbol REEP2
Gene ID (NCBI) 51308
RRIDAB_2238326
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for REEP2 antibody 15684-1-APDownload protocol
IHC protocol for REEP2 antibody 15684-1-APDownload protocol
IF protocol for REEP2 antibody 15684-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nature

ER proteins decipher the tubulin code to regulate organelle distribution

Authors - Pengli Zheng
mouseWB

Cell Metab

Receptor-Mediated ER Export of Lipoproteins Controls Lipid Homeostasis in Mice and Humans.

Authors - Xiao Wang
human,mouseWB

Cell Res

DNA damage triggers tubular endoplasmic reticulum extension to promote apoptosis by facilitating ER-mitochondria signaling.

Authors - Pengli Zheng
mouseIP

Mol Psychiatry

Dysfunctional tubular endoplasmic reticulum constitutes a pathological feature of Alzheimer's disease.

Authors - M G Sharoar
mouseWB

Hum Mol Genet

Reep1 null mice reveal a converging role for hereditary spastic paraplegia proteins in lipid droplet regulation.

Authors - Benoît Renvoisé
mouseWB

Hum Mol Genet

Transverse endoplasmic reticulum expansion in hereditary spastic paraplegia corticospinal axons.

Authors - Peng-Peng Zhu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Wanxia (Verified Customer) (07-16-2019)

Very good Reep2 antibody for WB and IF. It is so clean, almost no background.

  • Applications: Western Blot, Immunofluorescence,
  • Cell Tissue Type: Mouse Brain