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REEP2 Polyclonal antibody
REEP2 Polyclonal Antibody for WB, IHC, IF-P, FC (Intra), ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
WB, IHC, IF-P, FC (Intra), IP, ELISA
Conjugate
Unconjugated
Cat no : 15684-1-AP
Synonyms
Validation Data Gallery
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Tested Applications
| Positive WB detected in | mouse brain tissue, human heart tissue, mouse eye tissue, human skeletal muscle tissue, rat brain tissue |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse brain tissue |
| Positive FC (Intra) detected in | SH-SY5Y cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 11 publications below |
| IF | See 3 publications below |
| IP | See 1 publications below |
Product Information
15684-1-AP targets REEP2 in WB, IHC, IF-P, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, monkey |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | REEP2 fusion protein Ag8186 相同性解析による交差性が予測される生物種 |
| Full Name | receptor accessory protein 2 |
| Calculated molecular weight | 252 aa, 28 kDa |
| Observed molecular weight | 28 kDa |
| GenBank accession number | BC006218 |
| Gene symbol | REEP2 |
| Gene ID (NCBI) | 51308 |
| RRID | AB_2238326 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for REEP2 antibody 15684-1-AP | Download protocol |
| IHC protocol for REEP2 antibody 15684-1-AP | Download protocol |
| IF protocol for REEP2 antibody 15684-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Metab Receptor-Mediated ER Export of Lipoproteins Controls Lipid Homeostasis in Mice and Humans. | ||
Cell Res DNA damage triggers tubular endoplasmic reticulum extension to promote apoptosis by facilitating ER-mitochondria signaling. | ||
Mol Psychiatry Dysfunctional tubular endoplasmic reticulum constitutes a pathological feature of Alzheimer's disease. | ||
Hum Mol Genet Reep1 null mice reveal a converging role for hereditary spastic paraplegia proteins in lipid droplet regulation. | ||
Hum Mol Genet Transverse endoplasmic reticulum expansion in hereditary spastic paraplegia corticospinal axons. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Wanxia (Verified Customer) (07-16-2019) | Very good Reep2 antibody for WB and IF. It is so clean, almost no background.
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