Validation Data Gallery
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Tested Applications
| Positive WB detected in | mouse testis tissue, rat testis tissue |
| Positive IHC detected in | human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 4 publications below |
| IHC | See 1 publications below |
| IF | See 1 publications below |
Product Information
26650-1-AP targets REEP4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | REEP4 fusion protein Ag24569 相同性解析による交差性が予測される生物種 |
| Full Name | receptor accessory protein 4 |
| Calculated molecular weight | 29 kDa |
| Observed molecular weight | 29 kDa |
| GenBank accession number | BC013048 |
| Gene Symbol | REEP4 |
| Gene ID (NCBI) | 80346 |
| RRID | AB_2880589 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9H6H4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for REEP4 antibody 26650-1-AP | Download protocol |
| IHC protocol for REEP4 antibody 26650-1-AP | Download protocol |
| IF protocol for REEP4 antibody 26650-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Cell Biol Dynamic interaction of REEP5-MFN1/2 enables mitochondrial hitchhiking on tubular ER | ||
Hum Mol Genet Transverse endoplasmic reticulum expansion in hereditary spastic paraplegia corticospinal axons. | ||
J Biol Chem Comparative proximity biotinylation implicates the small GTPase RAB18 in sterol mobilization and biosynthesis | ||
Aging (Albany NY) Potential value of expression of receptor accessory protein 4 for evaluating the prognosis of lower-grade glioma patients | ||
J Cancer REEP4 as Potential Biomarker Associated with Predictive Prognosis and Immune Response in Kidney Clear Cell Carcinoma |