REEP4 Polyclonal antibody

REEP4 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 26650-1-AP

Synonyms

Receptor expression-enhancing protein 4, PP432, C8orf20


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Tested Applications

Positive WB detected inmouse testis tissue, rat testis tissue
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

26650-1-AP targets REEP4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen REEP4 fusion protein Ag24569 相同性解析による交差性が予測される生物種
Full Name receptor accessory protein 4
Calculated molecular weight 29 kDa
Observed molecular weight 29 kDa
GenBank accession numberBC013048
Gene symbol REEP4
Gene ID (NCBI) 80346
RRIDAB_2880589
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for REEP4 antibody 26650-1-APDownload protocol
IHC protocol for REEP4 antibody 26650-1-APDownload protocol
IF protocol for REEP4 antibody 26650-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nature

ER proteins decipher the tubulin code to regulate organelle distribution

Authors - Pengli Zheng
humanWB

J Cell Biol

Dynamic interaction of REEP5-MFN1/2 enables mitochondrial hitchhiking on tubular ER

Authors - Shue Chen
mouseWB

Hum Mol Genet

Transverse endoplasmic reticulum expansion in hereditary spastic paraplegia corticospinal axons.

Authors - Peng-Peng Zhu
humanIF

J Biol Chem

Comparative proximity biotinylation implicates the small GTPase RAB18 in sterol mobilization and biosynthesis

Authors - Robert S Kiss
humanIHC

Aging (Albany NY)

Potential value of expression of receptor accessory protein 4 for evaluating the prognosis of lower-grade glioma patients

Authors - Shuping Luo
humanWB

J Cancer

REEP4 as Potential Biomarker Associated with Predictive Prognosis and Immune Response in Kidney Clear Cell Carcinoma

Authors - Zixuan Chen