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REEP5 Polyclonal antibody

Name: REEP5 Polyclonal antibody
Brand: Proteintech
SKU: 14643-1-AP
Rating: 4.80 (5 reviews)

REEP5 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

Reviews (5)

Cat no : 14643-1-AP

Synonyms

TB2, Receptor expression-enhancing protein 5, Protein TB2, Polyposis locus protein 1, DP1

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICC

WB analysis using 14643-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 14643-1-AP (REEP5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 14643-1-AP
WB result of REEP5 antibody (14643-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-REEP5 transfected HeLa cells.
WB analysis of human liver using 14643-1-AP
human liver tissue were subjected to SDS PAGE followed by western blot with 14643-1-AP (REEP5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis using 14643-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 14643-1-AP (REEP5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 14643-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 14643-1-AP (REEP5 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of rat brain using 14643-1-AP
rat brain tissue were subjected to SDS PAGE followed by western blot with 14643-1-AP (REEP5 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 14643-1-AP
IP result of anti-REEP5 (IP:14643-1-AP, 3ug; Detection:14643-1-AP 1:1000) with HeLa cells lysate 2400ug.
IHC staining of human colon cancer using 14643-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14643-1-AP (REEP5 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 14643-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14643-1-AP (REEP5 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of A549 using 14643-1-AP
Immunofluorescent analysis of (4% PFA) fixed A549 cells using REEP5 antibody (14643-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L). F-actin is stained using CL555-phalloidin (red) and DNA is stained by DAPI (blue).
IF Staining of HeLa using 14643-1-AP
Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 14643-1-AP (REEP5 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Cat No. 14643-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HeLa cells, human liver tissue, mouse brain tissue, rat brain tissue, mouse heart tissue, rat heart tissue
Positive IP detected in	HeLa cells
Positive IHC detected in	human colon cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	A549 cells, HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:10000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:300-1:1200
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 5 publications below
WB	See 36 publications below
IHC	See 1 publications below
IF	See 20 publications below
IP	See 4 publications below

Product Information

14643-1-AP targets REEP5 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, monkey, xenopus
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	REEP5 fusion protein Ag6229 相同性解析による交差性が予測される生物種
Full Name	receptor accessory protein 5
Calculated molecular weight	21 kDa
Observed molecular weight	18-21 kDa
GenBank accession number	BC065926
Gene symbol	REEP5
Gene ID (NCBI)	7905
RRID	AB_2178440
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

REEP5 (receptor expression-enhancing protein 5), also named as C5orf18, DP1 or TB2, is a membrane protein belonging to DP1/Yop1p protein family. All REEP proteins contain a conserved TB2/DP1, HVA22 domain which is involved in intracellular trafficking and secretion (PMID: 19123125). REEP5 is expressed in circumvallate papillae and testis, and may have the function to influence functional cell surface expression of taste receptors (PMID: 16720576). REEP5 can interact with atlastins (ATL1 and ATL2) which are involved in modulating the structure of ER network (PMID: 19665976).

Protocols

Product Specific Protocols
WB protocol for REEP5 antibody 14643-1-AP	Download protocol
IHC protocol for REEP5 antibody 14643-1-AP	Download protocol
IF protocol for REEP5 antibody 14643-1-AP	Download protocol
IP protocol for REEP5 antibody 14643-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Nature ER proteins decipher the tubulin code to regulate organelle distribution Authors - Pengli Zheng View Article
human	WB	Cell A Genome-wide ER-phagy Screen Highlights Key Roles of Mitochondrial Metabolism and ER-Resident UFMylation. Authors - Jin Rui Liang KO Validated View Article
mouse	WB	Cell Metab Receptor-Mediated ER Export of Lipoproteins Controls Lipid Homeostasis in Mice and Humans. Authors - Xiao Wang View Article
human	WB	Nat Cell Biol Combinatorial selective ER-phagy remodels the ER during neurogenesis Authors - Melissa J Hoyer View Article
human,mouse	WB	Cell Res DNA damage triggers tubular endoplasmic reticulum extension to promote apoptosis by facilitating ER-mitochondria signaling. Authors - Pengli Zheng View Article
mouse	IP	Mol Psychiatry Dysfunctional tubular endoplasmic reticulum constitutes a pathological feature of Alzheimer's disease. Authors - M G Sharoar View Article

Reviews

Add a review

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH Andrea (Verified Customer) (05-10-2022)	Sharp and strong signal at 21 kDa with clear background. Applications: Western Blot Primary Antibody Dilution: 1:250 Cell Tissue Type: HeLa cell line REEP5 Antibody Western Blot validation (1:250 dilution) in HeLa cell line (Cat no:14643-1-AP)
FH Christine (Verified Customer) (11-10-2021)	Very good detection of endogenous REEP5 protein in human HEK293T and HeLa cell lines. Band at about 20 kDa (expected size for REEP5) Applications: Western Blot Primary Antibody Dilution: 1:2000 Cell Tissue Type: Cell culture of HeLa and HEK293T
FH Wei (Verified Customer) (09-22-2020)	The antibody gets sharp, single band on western blot. Applications: Western Blot Cell Tissue Type: Mouse heart
FH WEI (Verified Customer) (06-29-2020)	The staining pattern is consistent with cardiomyocytes like ER localization. Applications: Immunofluorescence, Primary Antibody Dilution: 1:3000 Cell Tissue Type: Mouse Heart
FH H (Verified Customer) (04-05-2020)	The antibody is very sensitive, such that 1 hour incubation using 1:3000 dilution worked perfectly. Applications: Western Blot Primary Antibody Dilution: 1:3000 Cell Tissue Type: HEK293T and HCT116 REEP5 Antibody Western Blot validation (1:3000 dilution) in HEK293T and HCT116 (Cat no:14643-1-AP)

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

REEP5 Polyclonal antibody

REEP5 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Validation Data Gallery

WB analysis using 14643-1-AP

WB analysis of HeLa using 14643-1-AP

WB analysis of human liver using 14643-1-AP

WB analysis using 14643-1-AP

WB analysis of mouse brain using 14643-1-AP

WB analysis of rat brain using 14643-1-AP

IP experiment of HeLa using 14643-1-AP

IHC staining of human colon cancer using 14643-1-AP

IHC staining of human colon cancer using 14643-1-AP

IF Staining of A549 using 14643-1-AP

IF Staining of HeLa using 14643-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

ER proteins decipher the tubulin code to regulate organelle distribution

A Genome-wide ER-phagy Screen Highlights Key Roles of Mitochondrial Metabolism and ER-Resident UFMylation.

Receptor-Mediated ER Export of Lipoproteins Controls Lipid Homeostasis in Mice and Humans.

Combinatorial selective ER-phagy remodels the ER during neurogenesis

DNA damage triggers tubular endoplasmic reticulum extension to promote apoptosis by facilitating ER-mitochondria signaling.

Dysfunctional tubular endoplasmic reticulum constitutes a pathological feature of Alzheimer's disease.

Reviews

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