Validation Data Gallery

Filter: AllWBIPIHCIF/ICCFC (Intra)

WB analysis using 14643-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 14643-1-AP (REEP5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 14643-1-AP
WB result of REEP5 antibody (14643-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-REEP5 transfected HeLa cells.
WB analysis using 14643-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 14643-1-AP (REEP5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 14643-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 14643-1-AP (REEP5 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of rat brain using 14643-1-AP
rat brain tissue were subjected to SDS PAGE followed by western blot with 14643-1-AP (REEP5 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of human liver using 14643-1-AP
human liver tissue were subjected to SDS PAGE followed by western blot with 14643-1-AP (REEP5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 14643-1-AP
IP result of anti-REEP5 (IP:14643-1-AP, 3ug; Detection:14643-1-AP 1:1000) with HeLa cells lysate 2400ug.
IHC staining of human colon cancer using 14643-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14643-1-AP (REEP5 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 14643-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14643-1-AP (REEP5 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of A549 using 14643-1-AP
Immunofluorescent analysis of (4% PFA) fixed A549 cells using REEP5 antibody (14643-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). F-actin is stained using CL555-phalloidin (red) and DNA is stained by DAPI (blue).
IF Staining of HeLa using 14643-1-AP
Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 14643-1-AP (REEP5 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
FC experiment of HeLa using 14643-1-AP
1x10^6 HeLa cells were intracellularly stained with 0.25 ug REEP5 Polyclonal antibody (14643-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Featured Product
KD/KO Validated

REEP5 Polyclonal antibody

REEP5 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Cat No. 14643-1-AP

Publications(51)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Synonyms

TB2, Receptor expression-enhancing protein 5, Protein TB2, Polyposis locus protein 1, DP1

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (51)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HeLa cells, human liver tissue, mouse brain tissue, rat brain tissue, mouse heart tissue, rat heart tissue
Positive IP detected in	HeLa cells
Positive IHC detected in	human colon cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	A549 cells, HeLa cells
Positive FC (Intra) detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:10000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:300-1:1200
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 5 publications below
WB	See 37 publications below
IHC	See 1 publications below
IF	See 22 publications below
IP	See 4 publications below

Product Information

14643-1-AP targets REEP5 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, monkey, xenopus
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	REEP5 fusion protein Ag6229 相同性解析による交差性が予測される生物種
Full Name	receptor accessory protein 5
Calculated molecular weight	21 kDa
Observed molecular weight	18-21 kDa
GenBank accession number	BC065926
Gene Symbol	REEP5
Gene ID (NCBI)	7905
RRID	AB_2178440
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q00765
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

REEP5 (receptor expression-enhancing protein 5), also named as C5orf18, DP1 or TB2, is a membrane protein belonging to DP1/Yop1p protein family. All REEP proteins contain a conserved TB2/DP1, HVA22 domain which is involved in intracellular trafficking and secretion (PMID: 19123125). REEP5 is expressed in circumvallate papillae and testis, and may have the function to influence functional cell surface expression of taste receptors (PMID: 16720576). REEP5 can interact with atlastins (ATL1 and ATL2) which are involved in modulating the structure of ER network (PMID: 19665976).

Protocols

Product Specific Protocols
WB protocol for REEP5 antibody 14643-1-AP	Download protocol
IHC protocol for REEP5 antibody 14643-1-AP	Download protocol
IF protocol for REEP5 antibody 14643-1-AP	Download protocol
IP protocol for REEP5 antibody 14643-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Nature ER proteins decipher the tubulin code to regulate organelle distribution Authors - Pengli Zheng View Article
human	WB	Cell A Genome-wide ER-phagy Screen Highlights Key Roles of Mitochondrial Metabolism and ER-Resident UFMylation. Authors - Jin Rui Liang KO Validated View Article
mouse	WB	Cell Metab Receptor-Mediated ER Export of Lipoproteins Controls Lipid Homeostasis in Mice and Humans. Authors - Xiao Wang View Article
human	WB	Nat Cell Biol Combinatorial selective ER-phagy remodels the ER during neurogenesis Authors - Melissa J Hoyer View Article
human	WB	Nat Struct Mol Biol Aurora kinase A-mediated phosphorylation triggers structural alteration of Rab1A to enhance ER complexity during mitosis Authors - Wei Zhang View Article
human	WB	Nat Commun AMFR-mediated Flavivirus NS2A ubiquitination subverts ER-phagy to augment viral pathogenicity Authors - Linliang Zhang View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 14643-1-AP

WB analysis of HeLa using 14643-1-AP

WB analysis using 14643-1-AP

WB analysis of mouse brain using 14643-1-AP

WB analysis of rat brain using 14643-1-AP

WB analysis of human liver using 14643-1-AP

IP experiment of HeLa using 14643-1-AP

IHC staining of human colon cancer using 14643-1-AP

IHC staining of human colon cancer using 14643-1-AP

IF Staining of A549 using 14643-1-AP

IF Staining of HeLa using 14643-1-AP

FC experiment of HeLa using 14643-1-AP

REEP5 Polyclonal antibody

REEP5 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

ER proteins decipher the tubulin code to regulate organelle distribution

A Genome-wide ER-phagy Screen Highlights Key Roles of Mitochondrial Metabolism and ER-Resident UFMylation.

Receptor-Mediated ER Export of Lipoproteins Controls Lipid Homeostasis in Mice and Humans.

Combinatorial selective ER-phagy remodels the ER during neurogenesis

Aurora kinase A-mediated phosphorylation triggers structural alteration of Rab1A to enhance ER complexity during mitosis

AMFR-mediated Flavivirus NS2A ubiquitination subverts ER-phagy to augment viral pathogenicity