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  • Featured Product
  • KD/KO Validated

REEP6 Polyclonal antibody

REEP6 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 12088-1-AP

Synonyms

Receptor expression-enhancing protein 6, Polyposis locus protein 1-like 1, DP1L1, C19orf32

Validation Data Gallery

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  • Western Blot (WB) analysis of BxPC-3 cells using REEP6 Polyclonal antibody (12088-1-AP)

    WB analysis of BxPC-3 using 12088-1-AP

    BxPC-3 cells were subjected to SDS PAGE followed by western blot with 12088-1-AP (REEP6 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using REEP6 Polyclonal antibody (12088-1-AP)

    WB analysis using 12088-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12088-1-AP (REEP6 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of BxPC-3 cells using REEP6 Polyclonal antibody (12088-1-AP)

    IP experiment of BxPC-3 using 12088-1-AP

    IP result of anti-REEP6 (IP:12088-1-AP, 4ug; Detection:12088-1-AP 1:600) with BxPC-3 cells lysate 2080 ug.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using REEP6 Polyclonal antibody (12088-1-AP)

    IHC staining of human pancreas cancer using 12088-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12088-1-AP (REEP6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using REEP6 Polyclonal antibody (12088-1-AP)

    IHC staining of human pancreas cancer using 12088-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12088-1-AP (REEP6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using REEP6 Polyclonal antibody (12088-1-AP)

    IF Staining of HepG2 using 12088-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using REEP6 antibody (12088-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of mouse olfactory epithelium tissue using REEP6 Polyclonal antibody (12088-1-AP)

    IF Staining of mouse olfactory epithelium tissue using 12088-1-AP

    Fluorescent IHC on 1%PLP fixed frozen mouse olfactory epithelium tissue of REEP6 antibody (12088-1-AP, 1:100). Excellent staining of Sus cells on the apical layer as well as their basal projections. By Dr. Brian Lin (Schwob Lab).

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Tested Applications

Positive WB detected inBxPC-3 cells, HepG2 cells, mouse liver tissue, rat liver tissue
Positive IP detected inBxPC-3 cells
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells, mouse olfactory epithelium tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12088-1-AP targets REEP6 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen REEP6 fusion protein Ag2723 相同性解析による交差性が予測される生物種
Full Name receptor accessory protein 6
Calculated molecular weight 201 aa, 22 kDa
Observed molecular weight 17-22 kDa
GenBank accession numberBC008201
Gene symbol REEP6
Gene ID (NCBI) 92840
RRIDAB_2178460
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for REEP6 antibody 12088-1-APDownload protocol
IHC protocol for REEP6 antibody 12088-1-APDownload protocol
IF protocol for REEP6 antibody 12088-1-APDownload protocol
IP protocol for REEP6 antibody 12088-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIP

Mol Psychiatry

Dysfunctional tubular endoplasmic reticulum constitutes a pathological feature of Alzheimer's disease.

Authors - M G Sharoar
View Article
ratWB

J Clin Invest

Nasal neuron PET imaging quantifies neuron generation and degeneration.

Authors - Genevieve C Van de Bittner
View Article
humanWB,IF

Am J Hum Genet

Mutations in REEP6 Cause Autosomal-Recessive Retinitis Pigmentosa.

Authors - Gavin Arno
View Article
mouseIF

Hum Mol Genet

REEP6 deficiency leads to retinal degeneration through disruption of ER homeostasis and protein trafficking.

Authors - Smriti A Agrawal
View Article