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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

REG Polyclonal antibody

REG Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 15850-1-AP

Synonyms

REG1 alpha, REG1A, Pancreatic thread protein, Pancreatic stone protein, P19

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using REG Polyclonal antibody (15850-1-AP)

    WB analysis using 15850-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15850-1-AP (REG antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using REG Polyclonal antibody (15850-1-AP)

    IHC staining of human stomach cancer using 15850-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 15850-1-AP (REG antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using REG Polyclonal antibody (15850-1-AP)

    IHC staining of human stomach cancer using 15850-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer slide using 15850-1-AP (REG antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using REG Polyclonal antibody (15850-1-AP)

    IHC staining of human pancreas cancer using 15850-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 15850-1-AP (REG antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using REG Polyclonal antibody (15850-1-AP)

    IHC staining of human pancreas cancer using 15850-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 15850-1-AP (REG antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using REG Polyclonal antibody (15850-1-AP)

    IHC staining of human stomach cancer using 15850-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 15850-1-AP (REG antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using REG Polyclonal antibody (15850-1-AP)

    IHC staining of human stomach cancer using 15850-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 15850-1-AP (REG antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inmouse pancreas tissue, rat pancreas tissue
Positive IHC detected inhuman stomach cancer tissue, human pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15850-1-AP targets REG in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen REG fusion protein Ag8640 相同性解析による交差性が予測される生物種
Full Name regenerating islet-derived 1 alpha
Calculated molecular weight 166 aa, 19 kDa
Observed molecular weight 19 kDa
GenBank accession numberBC005350
Gene symbol REG
Gene ID (NCBI) 5967
RRIDAB_2878192
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for REG antibody 15850-1-APDownload protocol
IHC protocol for REG antibody 15850-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Int Immunopharmacol

Protective and anti-inflammatory role of REG1A in inflammatory bowel disease induced by JAK/STAT3 signaling axis.

Authors - Hongli Mao
View Article
human,mouseWB,IF

Ann N Y Acad Sci

REG1A protects retinal photoreceptors from blue light damage

Authors - Ze-Hua Xu
View Article