• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

REL Monoclonal antibody

REL Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1F6A4

Cat no : 67747-1-Ig

Synonyms

C Rel, Proto oncogene c Rel, REL

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using REL Monoclonal antibody (67747-1-Ig)

    WB analysis using 67747-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67747-1-Ig (REL antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • Western Blot (WB) analysis of HEK-293 cells using REL Monoclonal antibody (67747-1-Ig)

    WB analysis of HEK-293 using 67747-1-Ig

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 67747-1-Ig (REL antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

☆大好評トライアルサイズ ¥20,000～！ >>> 一次抗体特別価格キャンペーン開催中！

Tested Applications

Positive WB detected inDaudi cells, HEK-293 cells, Jurkat cells, MOLT-4 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67747-1-Ig targets REL in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen REL fusion protein Ag26803 相同性解析による交差性が予測される生物種
Full Name v-rel reticuloendotheliosis viral oncogene homolog (avian)
Calculated molecular weight 69 kDa
Observed molecular weight68 kDa, 80 kDa
GenBank accession numberNM_001291746
Gene symbol REL
Gene ID (NCBI) 5966
RRIDAB_2918516
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

REL, also known as, Proto-oncogene c-Rel, is a 619 amino acid protein, which may play a role in differentiation and lymphopoiesis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. The NF-kappa-B heterodimer RELA/p65-c-Rel is a transcriptional activator. The full-length 80-kDa c-Rel was identified (PMID: 9857058).

Protocols

Product Specific Protocols
WB protocol for REL antibody 67747-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols