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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Renin Polyclonal antibody

Renin Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, monkey

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 29330-1-AP

Synonyms

Angiotensinogenase, FLJ10761, REN, Renin

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Renin Polyclonal antibody (29330-1-AP)

    WB analysis using 29330-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29330-1-AP (Renin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inCOS-7 cells, HepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

29330-1-AP targets Renin in WB, ELISA applications and shows reactivity with Human, monkey samples.

Tested Reactivity Human, monkey
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Renin fusion protein Ag31071 相同性解析による交差性が予測される生物種
Full Name renin
Calculated molecular weight 45 kDa
Observed molecular weight45 kDa
GenBank accession numberBC047752
Gene symbol Renin
Gene ID (NCBI) 5972
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Renin is an aspartic protease that consists of 2 homologous lobes, produced as pre-prorenin protein and transferred into the cisterns of the endoplasmic reticulum. Renin is mainly produced and released into circulation by the so-called juxtaglomerular epithelioid cells, located in the walls of renal afferent arterioles at the entrance of the glomerular capillary network (PMID: 16525949).REN has 2 isoforms produced by alternative splicing with a molecular weight of 45-47kDa.

Protocols

Product Specific Protocols
WB protocol for Renin antibody 29330-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols