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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

REPS2 Recombinant antibody

REPS2 Recombinant Antibody for IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

241136D3

Cat no : 84016-4-RR

Synonyms

REPS 2, RalBP1-interacting protein 2, RalBP1-associated Eps domain-containing protein 2, RalBP1 interacting protein 2, 241136D3

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of LNCaP cells using REPS2 Recombinant antibody (84016-4-RR)

    IF Staining of LNCaP using 84016-4-RR

    Immunofluorescent analysis of (4% PFA) fixed LNCaP cells using REPS2 antibody (84016-4-RR, Clone: 241136D3 ) at dilution of 1:100 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 84016-4-RR

    Affinity and Kinetic Characterization of 84016-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84016-4-RR against Human REPS2 were performed. The affinity constant is below 1 pM.

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Tested Applications

Positive IF/ICC detected inLNCaP cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 84016-4-RR is REPS2 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen REPS2 fusion protein Ag33764 相同性解析による交差性が予測される生物種
Full Name RALBP1 associated Eps domain containing 2
Calculated molecular weight72KD
GenBank accession numberNM_004726
Gene symbol REPS2
Gene ID (NCBI) 9185
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

REPS2 is part of a protein complex that regulates the endocytosis of growth factor receptors. The encoded protein directly interacts with a GTPase activating protein that functions downstream of the small G protein Ral. Its expression can negatively affect receptor internalization and inhibit growth factor signaling. There are 4 isoforms of the protein with two short (58 kDa) isoforms comprising 521 or 522 amino acid residues and two long (78 kDa) ones of 659 or 660 residues.

Protocols

Product Specific Protocols
IF protocol for REPS2 antibody 84016-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols