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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

REV1 Polyclonal antibody

REV1 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 17703-1-AP

Synonyms

AIBP80, DNA repair protein REV1, REV1, REV1 homolog (S. cerevisiae), REV1L

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using REV1 Polyclonal antibody (17703-1-AP)

    WB analysis using 17703-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 17703-1-AP (REV1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using REV1 Polyclonal antibody (17703-1-AP)

    IF Staining of U2OS using 17703-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using REV1 antibody (17703-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Tested Applications

Positive WB detected inHeLa cells
Positive IF/ICC detected inU2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

17703-1-AP targets REV1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen REV1 fusion protein Ag11975 相同性解析による交差性が予測される生物種
Full Name REV1 homolog (S. cerevisiae)
Calculated molecular weight 1114 aa, 122 kDa
Observed molecular weight140-150 kDa
GenBank accession numberBC037734
Gene symbol REV1
Gene ID (NCBI) 51455
RRIDAB_3085535
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Rev1 is a member of the TLS polymerase family and plays a key role in this mutagenic pathway, which allows the bypass of modified DNA bases and respectively, facilitates proliferation even in the presence of extensive DNA damage, such as during chemotherapy. Mutations in human REV1 have been detected in a minority of tumors, and single-nucleotide polymorphisms (SNPs) in the hREV1 gene have been linked to various types of human cancer.

Protocols

Product Specific Protocols
WB protocol for REV1 antibody 17703-1-APDownload protocol
IF protocol for REV1 antibody 17703-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

iScience

Distinct effects of sacituzumab govitecan and berzosertib on DNA damage response in ovarian cancer

Authors - Jayakumar R Nair
View Article