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Species Reactivity

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  • Featured Product
  • KD/KO Validated

RFC1 Polyclonal antibody

RFC1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 19159-1-AP

Synonyms

A1, A1 140 kDa subunit, Activator 1 140 kDa subunit, Activator 1 large subunit, Activator 1 subunit 1, DNA binding protein PO GA, MHCBFB, PO GA, RECC1, Replication factor C subunit 1, RF C 140 kDa subunit, RFC, RFC1, RFC140

Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using RFC1 Polyclonal antibody (19159-1-AP)

    WB analysis of Jurkat using 19159-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 19159-1-AP (RFC1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using RFC1 Polyclonal antibody (19159-1-AP)

    WB analysis of HeLa using 19159-1-AP

    WB result of RFC1 antibody (19159-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RFC1 transfected HeLa cells.

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Tested Applications

Positive WB detected inJurkat cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 19159-1-AP is RFC1 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RFC1 fusion protein Ag13585 相同性解析による交差性が予測される生物種
Full Name replication factor C (activator 1) 1, 145kDa
Calculated molecular weight 128 kDa
Observed molecular weight 128-150 kDa
GenBank accession numberBC051751
Gene symbol RFC1
Gene ID (NCBI) 5981
RRIDAB_2878574
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for RFC1 antibody 19159-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols