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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using RFC1 Polyclonal antibody (19159-1-AP)

    WB analysis of HeLa using 19159-1-AP

    WB result of RFC1 antibody (19159-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RFC1 transfected HeLa cells.

  • Western Blot (WB) analysis of Jurkat cells using RFC1 Polyclonal antibody (19159-1-AP)

    WB analysis of Jurkat using 19159-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 19159-1-AP (RFC1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  • KD/KO Validated

RFC1 Polyclonal antibody

RFC1 Polyclonal Antibody for WB, ELISA
Cat No. 19159-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

DNA binding protein PO GA, Activator 1 subunit 1, Activator 1 large subunit, Activator 1 140 kDa subunit, A1 140 kDa subunit

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inJurkat cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

19159-1-AP targets RFC1 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RFC1 fusion protein Ag13585 相同性解析による交差性が予測される生物種
Full Name replication factor C (activator 1) 1, 145kDa
Calculated molecular weight 128 kDa
Observed molecular weight 128-150 kDa
GenBank accession numberBC051751
Gene Symbol RFC1
Gene ID (NCBI) 5981
RRIDAB_2878574
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP35251
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Replication factor C subunit 1 (RFC1) is a pentameric DNA polymerase accessory protein involved in DNA replication and repair (PMID: 10051561). It was reported as a cause of cerebellar ataxia with neuropathy and vestibular areflexia syndrome (CANVAS) (PMID: 30926972).

Protocols

Product Specific Protocols
WB protocol for RFC1 antibody 19159-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols