Validation Data Gallery
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Tested Applications
| Positive WB detected in | HeLa cells, HepG2 cells, HEK-293T cells, Jurkat cells, A431 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
85148-1-RR targets RFC1 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | RFC1 fusion protein Ag13585 相同性解析による交差性が予測される生物種 |
| Full Name | replication factor C (activator 1) 1, 145kDa |
| Calculated molecular weight | 128 kDa |
| Observed molecular weight | 128-150 kDa |
| GenBank accession number | BC051751 |
| Gene Symbol | RFC1 |
| Gene ID (NCBI) | 5981 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P35251 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Replication factor C subunit 1 (RFC1) is a pentameric DNA polymerase accessory protein involved in DNA replication and repair (PMID: 10051561). It was reported as a cause of cerebellar ataxia with neuropathy and vestibular areflexia syndrome (CANVAS) (PMID: 30926972).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for RFC1 antibody 85148-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |