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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RFC2 Recombinant antibody (84892-1-RR)

    WB analysis using 84892-1-RR (same clone as 84892-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84892-1-RR (RFC2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84892-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP01625-2

    Sandwich ELISA standard curve of MP01625-2

    Sandwich ELISA standard curve of MP01625-2, Human RFC2 Recombinant Matched Antibody Pair - PBS only. 84892-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag0663. 84892-2-PBS was HRP conjugated as the detection antibody. Range: 0.156-10 ng/mL.

  • Sample test of MP01625-2

    Sample test of MP01625-2

    The mean RFC2 concentration was determined to be 55.11 ng/mL in HepG2 cell extract based on a 1.00 mg/mL extract load, 130.72 ng/mL in K-562 cell extract based on a 2.40 mg/mL extract load and 43.49 ng/mL in MCF-7 cell extract based on a 1.20 mg/mL extract load.

  • Immunohistochemistry (IHC) staining of mouse ovary tissue using RFC2 Recombinant antibody (84892-1-RR)

    IHC staining of mouse ovary using 84892-1-RR (same clone as 84892-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 84892-1-RR (RFC2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84892-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse ovary tissue using RFC2 Recombinant antibody (84892-1-RR)

    IHC staining of mouse ovary using 84892-1-RR (same clone as 84892-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 84892-1-RR (RFC2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84892-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of NIH/3T3 cells using RFC2 Recombinant antibody (84892-1-RR)

    IF Staining of NIH/3T3 using 84892-1-RR (same clone as 84892-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using RFC2 antibody (84892-1-RR, Clone: 242503E12 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84892-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using RFC2 Recombinant antibody (84892-1-RR)

    IF Staining of HeLa using 84892-1-RR (same clone as 84892-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RFC2 antibody (84892-1-RR, Clone: 242503E12 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84892-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using RFC2 Recombinant antibody (84892-1-RR)

    FC experiment of HeLa using 84892-1-RR (same clone as 84892-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug RFC2 Recombinant antibody (84892-1-RR, Clone:242503E12) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 84892-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01625-2

    Cytometric bead array standard curve of MP01625-2

    Cytometric bead array standard curve of MP01625-2, RFC2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84892-1-PBS. Detection antibody: 84892-2-PBS. Standard: Ag0663. Range: 0.625-80 ng/mL.

  • Affinity and Kinetic Characterization of 84892-1-RR

    Affinity and Kinetic Characterization of 84892-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84892-1-RR against Human RFC2 were performed. The affinity constant is 0.775 nM.

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RFC2 Recombinant antibody, PBS Only (Capture)

RFC2 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 84892-1-PBS
Clone No.242503E12

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

Replication factor C subunit 2, Activator 1 subunit 2, Activator 1 40 kDa subunit, A1 40 kDa subunit, A1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84892-1-PBS targets RFC2 as part of a matched antibody pair:

MP01625-2: 84892-1-PBS capture and 84892-2-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen RFC2 fusion protein Ag0663 相同性解析による交差性が予測される生物種
Full Name replication factor C (activator 1) 2, 40kDa
Calculated molecular weight 39 kDa
Observed molecular weight39 kDa
GenBank accession numberBC002813
Gene Symbol RFC2
Gene ID (NCBI) 5982
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP35250
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

RFC2, also named RFC40, belongs to the activator 1 small subunits family. It is a 39 kDa Activator 1 subunit. It binds ATP. The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins proliferating cell nuclear antigen (PCNA) and activator 1. RFC2 is a constitutively expressed protein essential for both DNA repair and replication. (PMID: 20660762).