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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RFC4 Monoclonal antibody

RFC4 Monoclonal Antibody for WB, FC (Intra), ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

1A6A11

Cat no : 68390-1-Ig

Synonyms

RFC37, RF-C 37 kDa subunit, A1, 1A6A11

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RFC4 Monoclonal antibody (68390-1-Ig)

    WB analysis using 68390-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68390-1-Ig (RFC4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

  • Flow cytometry (FC) experiment of HepG2 cells using RFC4 Monoclonal antibody (68390-1-Ig)

    FC experiment of HepG2 using 68390-1-Ig

    1x10^6 HepG2 cells were intracellularly stained with 0.4 ug RFC4 Monoclonal antibody (68390-1-Ig, Clone:1A6A11) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

Positive WB detected inU2OS cells, LNCaP cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, 4T1 cells
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68390-1-Ig targets RFC4 in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen RFC4 fusion protein Ag33295 相同性解析による交差性が予測される生物種
Full Name replication factor C (activator 1) 4, 37kDa
Calculated molecular weight 37 kDa
Observed molecular weight 37-40 kDa
GenBank accession numberBC017452
Gene symbol RFC4
Gene ID (NCBI) 5984
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for RFC4 antibody 68390-1-IgDownload protocol
FC protocol for RFC4 antibody 68390-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols