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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RFC5 Polyclonal antibody

RFC5 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 28671-1-AP

Synonyms

A1 36 kDa subunit, Activator 1 36 kDa subunit, Activator 1 subunit 5, Replication factor C subunit 5, RF C 36 kDa subunit, RFC36, RFC5

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RFC5 Polyclonal antibody (28671-1-AP)

    WB analysis using 28671-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28671-1-AP (RFC5 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using RFC5 Polyclonal antibody (28671-1-AP)

    IF Staining of HeLa using 28671-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RFC5 antibody (28671-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using RFC5 Polyclonal antibody (28671-1-AP)

    IF Staining of HeLa using 28671-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RFC5 antibody (28671-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inHCT 116 cells, U-251 cells, HEK-293T cells
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28671-1-AP targets RFC5 in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RFC5 fusion protein Ag29236 相同性解析による交差性が予測される生物種
Full Name replication factor C (activator 1) 5, 36.5kDa
Calculated molecular weight 36 aa, 5 kDa
GenBank accession numberBC001866
Gene symbol RFC5
Gene ID (NCBI) 5985
RRIDAB_2918189
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Replication factor C subunit 5 (RFC5), also known as activator 1, can recruit proliferating cell nuclear antigen(PCNA) onto the DNA polymerase S-phase checkpoint complex. In eukaryotes, RFC5 is involved in repairing mismatches, DNA double helix damage, nucleotide excision, and regulating the cell cycle(PMID: 30210909). RFC5 is significantly upregulated in cancer tissues or cells, and its expression is elevated with the cancer progression(PMID: 30214556).

Protocols

Product Specific Protocols
WB protocol for RFC5 antibody 28671-1-APDownload protocol
IF protocol for RFC5 antibody 28671-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols