Validation Data Gallery
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Tested Applications
| Positive WB detected in | HeLa cells, Jurkat cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 3 publications below |
Product Information
15813-1-AP targets RFK in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | RFK fusion protein Ag8591 相同性解析による交差性が予測される生物種 |
| Full Name | riboflavin kinase |
| Calculated molecular weight | 162 aa, 18 kDa |
| Observed molecular weight | 18 kDa |
| GenBank accession number | BC007069 |
| Gene Symbol | RFK |
| Gene ID (NCBI) | 55312 |
| RRID | AB_2178612 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q969G6 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Riboflavin kinase (RFK) is an essential enzyme that catalyzes the phosphorylation of riboflavin (vitamin B2) to form flavin mononucleotide (FMN), an obligatory step in vitamin B2 utilization and flavin cofactor synthesis(PMID:12623014). RFK is rate-limiting in the synthesis of FAD, an essential prosthetic group of NADPH oxidase.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for RFK antibody 15813-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Transl Psychiatry Long non-coding RNA Neat1 regulates adaptive behavioural response to stress in mice. | ||
Eur J Immunol Riboflavin (vitamin B2 ) deficiency impairs Nox2 priming and defense against Listeria monocytogenes. | ||
Adv Sci (Weinh) Biomimetic Remodeling of Microglial Riboflavin Metabolism Ameliorates Cognitive Impairment by Modulating Neuroinflammation |