Validation Data Gallery
![Dilution series of mCherry in HEK 293T
cell lysate
Primary antibody: [6G6] 1:1,000
Secondary antibody: goat anti-mouse
Alexa Fluor® 647 1:1,000](https://www.ptglab.com/products/pictures/Result-6g6.png "Dilution series of mCherry in HEK 293T
cell lysate
Primary antibody: [6G6] 1:1,000
Secondary antibody: goat anti-mouse
Alexa Fluor® 647 1:1,000")
Product Information
Mouse monoclonal antibody [6G6] to Red Fluorescent Proteins
| Description | RFP mouse monoclonal [6G6] for WB |
| Applications | Western blotting |
| Specificity/Target | Tested on mOrange, OFP, DsRed, mCherry, mRFP, mRFPRuby, mScarlet, mScarlet-I, tdTomato, mPlum. For the complete list, please click here: Fluorescent protein specificity table antibodies |
| Recommended Dilution | Western Blot: 1:2,000 |
| Host/IsoType | Mouse/IgG2c |
| Class | Monoclonal |
| Type | Primary Antibody |
| Immunogen | Red fluorescent protein (RFP) |
| UniProtKB | Q9U6Y8 |
| Clone No. | 6G6 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A or G purified |
| RRID | AB_2631395 |
| Storage Buffer | PBS, 50% glycerol, preservative: 0.09% sodium azide. |
| Storage Condition | Shipped on ice. Store at -20°C/-4°F. Stable for 1 year after shipment. |
| Size | 20 μL; 150 μL |
| Background | Red fluorescent proteins (RFPs) is a collective term referring to a heterogenous group of red chromophore-carrying proteins, originating from various species and forming different protein lineages. The original RFP (dsRed) is a 225 amino acid fluorescent protein (25.9 kDa) derived from Discosoma sp.. It emits red light with a peak wavelength of 593 nm upon excitation by green light (excitation peak at 558 nm). When fused with other proteins, RFP serves as a versatile reporter protein e.g. for quantifying expression levels or facilitates visualization of subcellular localization through fluorescence microscopy. This antibody is a mouse (IgG2c) monoclonal antibody raised against RFP, reactive to a variety of RFP derivates, including mOrange, OFP, DsRed, mCherry, mRFP, mRFPRuby, mScarlet, mScarlet-I, tdTomato, and mPlum. |
Publications
| Application | Title |
|---|---|
Nature POLAR-guided signalling complex assembly and localization drive asymmetric cell division. | |
Cell Deficient Endoplasmic Reticulum-Mitochondrial Phosphatidylserine Transfer Causes Liver Disease. | |
Cell Formation of NPR1 Condensates Promotes Cell Survival during the Plant Immune Response. |