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Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of rat testis tissue using RFPL4B-Specific Polyclonal antibody (20207-1-AP)

    IHC staining of rat testis using 20207-1-AP

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 20207-1-AP (RFPL4B-Specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using RFPL4B-Specific Polyclonal antibody (20207-1-AP)

    IF Staining of A549 using 20207-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using RFPL4B-Specific antibody (20207-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

RFPL4B-Specific Polyclonal antibody

RFPL4B-Specific Polyclonal Antibody for IHC, IF/ICC, ELISA
Cat No. 20207-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

IHC, IF/ICC, ELISA

Synonyms

RFPL4B, RNF211, RING finger protein 211, Ret finger protein-like 4B, ret finger protein like 4B

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IHC detected inrat testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20207-1-AP targets RFPL4B-Specific in IHC, IF/ICC, ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name ret finger protein-like 4B
Calculated molecular weight 30 kDa
GenBank accession numberNM_001013734
Gene Symbol RFPL4B
Gene ID (NCBI) 442247
RRIDAB_3669329
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6ZWI9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RFPL4B also named as RNF211. This antibody is specific to RFPL4B. RFPL4B may enable metal ion binding activity.

Protocols

Product Specific Protocols
IHC protocol for RFPL4B-Specific antibody 20207-1-APDownload protocol
IF protocol for RFPL4B-Specific antibody 20207-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols