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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RFTN2 Polyclonal antibody

RFTN2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 17854-1-AP

Synonyms

C2orf11, Raft linking protein 2, Raftlin 2, raftlin family member 2, RFTN2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RFTN2 Polyclonal antibody (17854-1-AP)

    WB analysis using 17854-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 17854-1-AP (RFTN2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse testis tissue using RFTN2 Polyclonal antibody (17854-1-AP)

    WB analysis of mouse testis using 17854-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 17854-1-AP (RFTN2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using RFTN2 Polyclonal antibody (17854-1-AP)

    WB analysis of mouse brain using 17854-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 17854-1-AP (RFTN2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using RFTN2 Polyclonal antibody (17854-1-AP)

    IHC staining of human liver cancer using 17854-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17854-1-AP (RFTN2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using RFTN2 Polyclonal antibody (17854-1-AP)

    IHC staining of human liver cancer using 17854-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17854-1-AP (RFTN2 Antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using RFTN2 Polyclonal antibody (17854-1-AP)

    IHC staining of human liver cancer using 17854-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17854-1-AP (RFTN2 Antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using RFTN2 Polyclonal antibody (17854-1-AP)

    IHC staining of human liver cancer using 17854-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17854-1-AP (RFTN2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inmouse lung tissue, mouse brain tissue, mouse testis tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17854-1-AP targets RFTN2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RFTN2 fusion protein Ag12307 相同性解析による交差性が予測される生物種
Full Name raftlin family member 2
Calculated molecular weight 501 aa, 56 kDa
Observed molecular weight 56-60 kDa
GenBank accession numberBC111069
Gene symbol RFTN2
Gene ID (NCBI) 130132
RRIDAB_2300934
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for RFTN2 antibody 17854-1-APDownload protocol
IHC protocol for RFTN2 antibody 17854-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols