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  • KD/KO Validated

RFX1 Polyclonal antibody

RFX1 Polyclonal Antibody for WB, IP, ELISA
Cat No. 26859-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF, IP, ELISA

EF C, Enhancer factor C, Regulatory factor X 1, RFX, RFX1, Transcription factor RFX1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration:  150 μL, 500 ug/ml
20 μL, 500 ug/ml
150 μL, 500 ug/ml

¥67,000/ 150 μL

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テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive WB detected inJurkat cells, HL-60 cells
Positive IP detected inJurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

26859-1-AP targets RFX1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RFX1 fusion protein Ag25442 相同性解析による交差性が予測される生物種
Full Name regulatory factor X, 1 (influences HLA class II expression)
Calculated molecular weight 105 kDa
Observed molecular weight 135 kDa
GenBank accession numberBC049826
Gene Symbol RFX1
Gene ID (NCBI) 5989
RRIDAB_2880659
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP22670
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RFX1, also named as MHC class II regulatory factor RFX1, is a 979 amino acid protein which contains 1 RFX-type winged-helix DNA-binding domain and belongs to the RFX family. RFX1 can binds DNA as a homodimer and heterodimerizes with RFX2 and RFX3. RFX1 as a regulatory factor is essential for MHC class II genes expression. RFX1 protein is modified after translation and its molecular weight could be migrate to 130-140 kDa (PMID: 20189986 ).

Protocols

Product Specific Protocols
WB protocol for RFX1 antibody 26859-1-APDownload protocol
IP protocol for RFX1 antibody 26859-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouse,humanIF,WB

Int J Biol Sci

Hypoxia-induced exosomes facilitate lung pre-metastatic niche formation in hepatocellular carcinoma through the miR-4508-RFX1-IL17A-p38 MAPK-NF-κB pathway

Authors - Wentao Jia
  • KD Validated
human,mouseIHC

Ren Fail

Proteomics reveals the key transcription-related factors mediating obstructive nephropathy in pediatric patients and mice

Authors - Hualin Cao
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