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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RFX3 Polyclonal antibody

RFX3 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ChIP, ELISA

Conjugate

Unconjugated

Cat no : 14784-1-AP

Synonyms

Regulatory factor X 3, RFX3, Transcription factor RFX3

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using RFX3 Polyclonal antibody (14784-1-AP)

    WB analysis of HeLa using 14784-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14784-1-AP (RFX3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using RFX3 Polyclonal antibody (14784-1-AP)

    WB analysis of HeLa using 14784-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 14784-1-AP (RFX3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using RFX3 Polyclonal antibody (14784-1-AP)

    WB analysis of K-562 using 14784-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 14784-1-AP (RFX3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using RFX3 Polyclonal antibody (14784-1-AP)

    IP experiment of HeLa using 14784-1-AP

    IP result of anti-RFX3 (IP:14784-1-AP, 4ug; Detection:14784-1-AP 1:500) with HeLa cells lysate 1400ug.

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Tested Applications

Positive WB detected inHeLa cells, K-562 cells
Positive IP detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14784-1-AP targets RFX3 in WB, IP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RFX3 fusion protein Ag6413 相同性解析による交差性が予測される生物種
Full Name regulatory factor X, 3 (influences HLA class II expression)
Calculated molecular weight 84 kDa
Observed molecular weight 70-79 kDa
GenBank accession numberBC067778
Gene symbol RFX3
Gene ID (NCBI) 5991
RRIDAB_2178917
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for RFX3 antibody 14784-1-APDownload protocol
IP protocol for RFX3 antibody 14784-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratChIP

Nat Commun

Distinct regulation of hippocampal neuroplasticity and ciliary genes by corticosteroid receptors.

Authors - Karen R Mifsud
View Article
mouseWB

Aging (Albany NY)

RNA-seq analysis of the key long noncoding RNAs and mRNAs related to cognitive impairment after cardiac arrest and cardiopulmonary resuscitation.

Authors - Chan Chen
View Article