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  • Featured Product
  • KD/KO Validated

RFX6 Polyclonal antibody, PBS Only

RFX6 Polyclonal Antibody for WB, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

Cat no : 22551-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of Caco-2 cells using RFX6 Polyclonal antibody (22551-1-AP)

    WB analysis of Caco-2 using 22551-1-AP (same clone as 22551-1-PBS)

    Caco-2 cells were subjected to SDS PAGE followed by western blot with 22551-1-AP (RFX6 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22551-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Caco-2 cells using RFX6 Polyclonal antibody (22551-1-AP)

    WB analysis of Caco-2 using 22551-1-AP (same clone as 22551-1-PBS)

    Caco-2 cells were subjected to SDS PAGE followed by western blot with 22551-1-AP (RFX6 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22551-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

22551-1-PBS targets RFX6 in WB, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RFX6 fusion protein Ag18235 相同性解析による交差性が予測される生物種
Full Name regulatory factor X, 6
Calculated molecular weight 928 aa, 102 kDa
Observed molecular weight 102 kDa
GenBank accession numberBC039248
Gene symbol RFX6
Gene ID (NCBI) 222546
RRIDAB_2879120
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

The RFX proteins represent an essential class II transcription factor family that share several conserved regions, including a centrally located DNA-binding domain (DBD) and a C-terminal D region that facilitates dimerization. RFX6, also known as RFXDC1, is a 928 amino acid nuclear protein that, via interactions with other RFX proteins, can bind DNA and is thought to activate the transcription of target genes. RFX6 is specifically expressed in pancreas, small intestine and colon. Mutations in the gene encoding RFX6 is the cause of the Mitchell-Riley syndrome (MIRIS), which is characterized by neonatal diabetes, duodenal and jejunal atresia, a hypoplastic or annular pancreas and absent gallbladder.