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RGC32 Polyclonal antibody

RGC32 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 19556-1-AP

Synonyms

C13orf15, bA157L14.2, RGCC, RGC-32, RGC 32


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Tested Applications

Positive WB detected inmouse brain tissue, mouse liver tissue
Positive IHC detected inmouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inCaco-2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

19556-1-AP targets RGC32 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name chromosome 13 open reading frame 15
Calculated molecular weight 15 kDa
Observed molecular weight13-15 kDa
GenBank accession numberNM_014059
Gene symbol RGC32
Gene ID (NCBI) 28984
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RGC32, also named as C13orf15, modulates the activity of cell cycle-specific kinases. RGC32 enhances CDC2 activity. RGC32 may contribute to the regulation of the cell cycle. And it inhibits growth of glioma cells by promoting arrest of mitotic progression at the G2/M transition. The antibody can recognize these 2 isoforms of RGC32.

Protocols

Product Specific Protocols
WB protocol for RGC32 antibody 19556-1-APDownload protocol
IHC protocol for RGC32 antibody 19556-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IF

J Immunol

RGC32 Promotes Bleomycin-Induced Systemic Sclerosis in a Murine Disease Model by Modulating Classically Activated Macrophage Function.

Authors - Chenming Sun
  • KO Validated