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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RGL3 Polyclonal antibody

RGL3 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 26626-1-AP

Synonyms

RalGDS like 3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RGL3 Polyclonal antibody (26626-1-AP)

    WB analysis using 26626-1-AP

    HepG2 cells, RT-4 cells, Caco-2 cells lysates were subjected to SDS PAGE followed by western blot with 26626-1-AP (RGL3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inRT-4 cells, HepG2 cells, Caco-2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

26626-1-AP targets RGL3 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RGL3 fusion protein Ag24329 相同性解析による交差性が予測される生物種
Full Name ral guanine nucleotide dissociation stimulator-like 3
Calculated molecular weight 710 aa, 78 kDa
Observed molecular weight78 kDa
GenBank accession numberBC101756
Gene symbol RGL3
Gene ID (NCBI) 57139
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RGL3 is a guanine nucleotide exchange factor. RGL3 mRNA is commonly detectable in several visceral organs (e.g. kidney, heart, liver, and lung) in the mouse and human. The RGL3 protein mainly localizes in the cytoplasm when expressed in fibroblasts. Yeast two-hybrid assay indicated that RGL3 could interact with Rap1, Rap2, H-Ras, N-Ras, and R-Ras but failed to interact efficiently with Ral and Rho (PMID: 17382517).

Protocols

Product Specific Protocols
WB protocol for RGL3 antibody 26626-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols