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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ARHGEF28 Polyclonal antibody (17144-1-AP)

    WB analysis using 17144-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17144-1-AP (RGNEF antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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ARHGEF28 Polyclonal antibody

ARHGEF28 Polyclonal Antibody for WB, ELISA
Cat No. 17144-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

RGNEF, Rho guanine nucleotide exchange factor 28, p190-RhoGEF, p190RhoGEF, p190 RhoGEF

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, Jurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17144-1-AP targets ARHGEF28 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ARHGEF28 fusion protein Ag10915 相同性解析による交差性が予測される生物種
Full Name Rho-guanine nucleotide exchange factor
Calculated molecular weight 192 kDa
Observed molecular weight192 kDa
GenBank accession numberBC012946
Gene Symbol RGNEF
Gene ID (NCBI) 64283
RRIDAB_3669262
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8N1W1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ARHGEF28, also known as RGNEF or p190RhoGEF, is a 190 kDa RNA binding protein (RBP) that contains a Dbl/PH domain capable of RhoA activation. ARHGEF28 interacts with low molecular weight neurofilament mRNA and may be involved in the formation of amyotrophic lateral sclerosis neurofilament aggregates.

Protocols

Product Specific Protocols
WB protocol for ARHGEF28 antibody 17144-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols