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RGS1 Polyclonal antibody

RGS1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12547-1-AP

Synonyms

IER1, Early response protein 1R20, BL34, B-cell activation protein BL34, B cell activation protein BL34

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RGS1 Polyclonal antibody (12547-1-AP)

    WB analysis using 12547-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12547-1-AP (RGS1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using RGS1 Polyclonal antibody (12547-1-AP)

    WB analysis using 12547-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12547-1-AP (RGS1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using RGS1 Polyclonal antibody (12547-1-AP)

    WB analysis of HepG2 using 12547-1-AP

    WB result of RGS1 antibody (12547-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-RGS1 transfected HepG2 cells.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using RGS1 Polyclonal antibody (12547-1-AP)

    IHC staining of human stomach cancer using 12547-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 12547-1-AP (RGS1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Sodium Citrate buffer (pH 6.0).

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Tested Applications

Positive WB detected inHL-60 cells, mouse small intestine tissue, HepG2 cells, NK-92 cells, rat small intestine tissue
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12547-1-AP targets RGS1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RGS1 fusion protein Ag3315 相同性解析による交差性が予測される生物種
Full Name regulator of G-protein signaling 1
Calculated molecular weight 196 aa, 22 kDa
Observed molecular weight 30 kDa
GenBank accession numberBC015510
Gene symbol RGS1
Gene ID (NCBI) 5996
RRIDAB_3085397
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RGS1 (Regulator of G-protein signaling 1) is also named as 1R20, BL34 and IER1. RGS1 encodes a member of the regulator of G-protein signaling family, which can act as a GTPase-activating protein (GAP), increasing the rate of conversion of the GTP to GDP, driving G-protein into its inactive GDP-bound form, hence attenuating or turning off G-protein-coupled receptor signaling (PMID: 8774882). RGS1 is highly expressed in immune cells including T cells, B cells, natural killer (NK) cells, dendritic cells and monocytes, suggesting a role for RGS1 in immune cell regulation (PMID: 34956194). RGS1 was highly expressed in tumor tissues and correlated with shorter overall survival, which also appeared in several previous studies, including multiple myeloma, melanoma, nonsmall cell lung cancer, gastric cancer , diffuse large B-cell lymphoma (PMID: 34956194). RGS1 protein, located at the cytoplasm and membrane, is enriched in tumor tissues compared with normal tissues. RGS1 was identified as a HIF-dependent hypoxia target that dampens cell migration and signal transduction (PMID: 24686421).

Protocols

Product Specific Protocols
WB protocol for RGS1 antibody 12547-1-APDownload protocol
IHC protocol for RGS1 antibody 12547-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols