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  • Featured Product
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RGS1 Polyclonal antibody

RGS1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12547-1-AP

Synonyms

IER1, Early response protein 1R20, BL34, B-cell activation protein BL34, B cell activation protein BL34

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RGS1 Polyclonal antibody (12547-1-AP)

    WB analysis using 12547-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12547-1-AP (RGS1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using RGS1 Polyclonal antibody (12547-1-AP)

    WB analysis using 12547-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12547-1-AP (RGS1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using RGS1 Polyclonal antibody (12547-1-AP)

    WB analysis of HepG2 using 12547-1-AP

    WB result of RGS1 antibody (12547-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-RGS1 transfected HepG2 cells.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using RGS1 Polyclonal antibody (12547-1-AP)

    IHC staining of human stomach cancer using 12547-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 12547-1-AP (RGS1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Sodium Citrate buffer (pH 6.0).

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Tested Applications

Positive WB detected inHL-60 cells, mouse small intestine tissue, HepG2 cells, NK-92 cells, rat small intestine tissue
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12547-1-AP targets RGS1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RGS1 fusion protein Ag3315 相同性解析による交差性が予測される生物種
Full Name regulator of G-protein signaling 1
Calculated molecular weight 196 aa, 22 kDa
Observed molecular weight 30 kDa
GenBank accession numberBC015510
Gene symbol RGS1
Gene ID (NCBI) 5996
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RGS1 (Regulator of G-protein signaling 1) is also named as 1R20, BL34 and IER1. RGS1 encodes a member of the regulator of G-protein signaling family, which can act as a GTPase-activating protein (GAP), increasing the rate of conversion of the GTP to GDP, driving G-protein into its inactive GDP-bound form, hence attenuating or turning off G-protein-coupled receptor signaling (PMID: 8774882). RGS1 is highly expressed in immune cells including T cells, B cells, natural killer (NK) cells, dendritic cells and monocytes, suggesting a role for RGS1 in immune cell regulation (PMID: 34956194). RGS1 was highly expressed in tumor tissues and correlated with shorter overall survival, which also appeared in several previous studies, including multiple myeloma, melanoma, nonsmall cell lung cancer, gastric cancer , diffuse large B-cell lymphoma (PMID: 34956194). RGS1 protein, located at the cytoplasm and membrane, is enriched in tumor tissues compared with normal tissues. RGS1 was identified as a HIF-dependent hypoxia target that dampens cell migration and signal transduction (PMID: 24686421).

Protocols

Product Specific Protocols
WB protocol for RGS1 antibody 12547-1-APDownload protocol
IHC protocol for RGS1 antibody 12547-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols