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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RGS11 Polyclonal antibody

RGS11 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 26132-1-AP

Synonyms

RGS11, RS11

Validation Data Gallery

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  • Western Blot (WB) analysis of HL-60 cells using RGS11 Polyclonal antibody (26132-1-AP)

    WB analysis of HL-60 using 26132-1-AP

    HL-60 cells were subjected to SDS PAGE followed by western blot with 26132-1-AP (RGS11 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat brain tissue using RGS11 Polyclonal antibody (26132-1-AP)

    WB analysis of rat brain using 26132-1-AP

    rat brain tissue were subjected to SDS PAGE followed by western blot with 26132-1-AP (RGS11 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using RGS11 Polyclonal antibody (26132-1-AP)

    IHC staining of human lung cancer using 26132-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 26132-1-AP (RGS11 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using RGS11 Polyclonal antibody (26132-1-AP)

    IHC staining of human lung cancer using 26132-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 26132-1-AP (RGS11 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inHL-60 cells, rat brain tissue
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 26132-1-AP is RGS11 Fusion Protein expressed in E. coli.

Tested Reactivity human, rat, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RGS11 fusion protein Ag23562 相同性解析による交差性が予測される生物種
Full Name regulator of G-protein signaling 11
Observed molecular weight 53 kDa
GenBank accession numberBC153019
Gene symbol RGS11
Gene ID (NCBI) 8786
RRIDAB_2880394
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for RGS11 antibody 26132-1-APDownload protocol
IHC protocol for RGS11 antibody 26132-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols