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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RGS12 Polyclonal antibody

RGS12 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 29803-1-AP

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RGS12 Polyclonal antibody (29803-1-AP)

    WB analysis using 29803-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29803-1-AP (RGS12 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using RGS12 Polyclonal antibody (29803-1-AP)

    WB analysis using 29803-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29803-1-AP (RGS12 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of rat cerebellum tissue using RGS12 Polyclonal antibody (29803-1-AP)

    IHC staining of rat cerebellum using 29803-1-AP

    Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 29803-1-AP (RGS12 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of PC-3 cells using RGS12 Polyclonal antibody (29803-1-AP)

    IF Staining of PC-3 using 29803-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed PC-3 cells using RGS12 antibody (29803-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inHepG2 cells, A431 cells, SH-SY5Y cells, THP-1 cells, NIH/3T3 cells
Positive IHC detected inrat cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inPC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

29803-1-AP targets RGS12 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RGS12 fusion protein Ag30731 相同性解析による交差性が予測される生物種
Full Name regulator of G-protein signaling 12
Calculated molecular weight 156 kDa
Observed molecular weight140-150 kDa
GenBank accession numberNM_198229
Gene symbol RGS12
Gene ID (NCBI) 6002
RRIDAB_2935482
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Regulator of G-protein signaling 12 (RGS12) regulates G protein-coupled receptor signaling cascades. Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits, thereby driving them into their inactive GDP-bound form. This antibody can be detected as 140-150 kDa.

Protocols

Product Specific Protocols
WB protocol for RGS12 antibody 29803-1-APDownload protocol
IHC protocol for RGS12 antibody 29803-1-APDownload protocol
IF protocol for RGS12 antibody 29803-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols