Validation Data Gallery
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Tested Applications
Positive WB detected in | A431 cells, HepG2 cells, SH-SY5Y cells, THP-1 cells, NIH/3T3 cells, C6 cells, mouse brain tissue |
Positive IF/ICC detected in | PC-3 cells |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:5000-1:50000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
85057-2-RR targets RGS12 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Immunogen | RGS12 fusion protein Ag30731 相同性解析による交差性が予測される生物種 |
Full Name | regulator of G-protein signaling 12 |
Calculated molecular weight | 156 kDa |
Observed molecular weight | 140-150 kDa |
GenBank accession number | NM_198229 |
Gene Symbol | RGS12 |
Gene ID (NCBI) | 6002 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | O14924 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
Product Specific Protocols | |
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WB protocol for RGS12 antibody 85057-2-RR | Download protocol |
IF protocol for RGS12 antibody 85057-2-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |