Validation Data Gallery
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Tested Applications
| Positive WB detected in | A431 cells, HepG2 cells, SH-SY5Y cells, THP-1 cells, NIH/3T3 cells, C6 cells, mouse brain tissue |
| Positive IF/ICC detected in | PC-3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
85057-2-RR targets RGS12 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | RGS12 fusion protein Ag30731 相同性解析による交差性が予測される生物種 |
| Full Name | regulator of G-protein signaling 12 |
| Calculated molecular weight | 156 kDa |
| Observed molecular weight | 140-150 kDa |
| GenBank accession number | NM_198229 |
| Gene Symbol | RGS12 |
| Gene ID (NCBI) | 6002 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | O14924 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for RGS12 antibody 85057-2-RR | Download protocol |
| IF protocol for RGS12 antibody 85057-2-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |