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KD/KO Validated

RGS14 Polyclonal antibody

Name: RGS14 Polyclonal antibody
Brand: Proteintech
SKU: 16258-1-AP

RGS14 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (2)

Applications

WB, IHC, IP, CoIP, ELISA, IF

Conjugate

Unconjugated

Publications

Review Product

Cat no : 16258-1-AP

Synonyms

RGS14

Validation Data Gallery

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AllWBIPIHC

WB analysis using 16258-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 16258-1-AP (RGS14 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 16258-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 16258-1-AP (RGS14 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 16258-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 16258-1-AP (RGS14 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse spleen using 16258-1-AP
mouse spleen tissue were subjected to SDS PAGE followed by western blot with 16258-1-AP (RGS14 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse testis using 16258-1-AP
mouse testis tissue were subjected to SDS PAGE followed by western blot with 16258-1-AP (RGS14 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
IP experiment of mouse brain using 16258-1-AP
IP result of anti-RGS14 (IP:16258-1-AP, 3ug; Detection:16258-1-AP 1:1000) with mouse brain tissue lysate 5000ug.
IHC staining of mouse brain using 16258-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16258-1-AP (RGS14 antibody) at dilution of 1:5000 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 16258-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16258-1-AP (RGS14 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Cat No. 16258-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HeLa cells, mouse testis tissue, mouse brain tissue, mouse spleen tissue, HepG2 cells, HuH-7 cells, mouse liver tissue
Positive IP detected in	mouse brain tissue
Positive IHC detected in	mouse brain tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:2500-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 2 publications below
WB	See 5 publications below
IHC	See 3 publications below
IF	See 4 publications below
IP	See 1 publications below
CoIP	See 1 publications below

Product Information

16258-1-AP targets RGS14 in WB, IHC, IP, CoIP, ELISA, IF applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse, rat, monkey
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	RGS14 fusion protein Ag9292 相同性解析による交差性が予測される生物種
Full Name	regulator of G-protein signaling 14
Calculated molecular weight	566 aa, 61 kDa
Observed molecular weight	60-65 kDa
GenBank accession number	BC014094
Gene symbol	RGS14
Gene ID (NCBI)	10636
RRID	AB_2179918
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

RGS14, a member of the R12 subfamily of RGS proteins, is highly expressed in the brain and is a natural suppressor of CA2 hippocampal synaptic plasticity and learning and memory. RGS14 was first identified as a complex scaffolding protein with an unconventional domain structure that allows it to interact with various protein binding partners. RGS14 contains one RGS domain, two Raf-like Ras-binding domains (RBDs), and one GoLoco domain. The protein attenuates the signaling activity of G-proteins by binding, through its GoLoco domain, to specific types of activated, GTP-bound G alpha subunits. Acting as a GTPase activating protein (GAP), the protein increases the rate of conversion of the GTP to GDP.

Protocols

Product Specific Protocols
WB protocol for RGS14 antibody 16258-1-AP	Download protocol
IHC protocol for RGS14 antibody 16258-1-AP	Download protocol
IP protocol for RGS14 antibody 16258-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human,mouse	WB,CoIP	Mol Metab Hepatic Regulator of G protein Signaling 14 Ameliorates NAFLD through Activating cAMP-AMPK Signaling by Targeting Giα1/3 Authors - Junyong Wang KO Validated View Article
human,monkey	WB,IHC	Brain Struct Funct Regulator of G protein signaling 14 (RGS14) is expressed pre- and postsynaptically in neurons of hippocampus, basal ganglia, and amygdala of monkey and human brain. Authors - Katherine E Squires View Article
rat	IHC	Exp Neurol Modelling cognitive deficits in Parkinson's disease: Is CA2 a gateway for hippocampal synucleinopathy? Authors - Elif Cinar View Article
human	WB, IF	J Biol Chem Human genetic variants disrupt RGS14 nuclear shuttling and regulation of LTP in hippocampal neurons. Authors - Katherine E Squires View Article
human	WB,IF	J Biol Chem RGS14 regulates hormone-sensitive NPT2A-mediated renal phosphate uptake via binding to the NHERF1 scaffolding protein. Authors - Peter A Friedman View Article
mouse	IP	J Biol Chem 14-3-3γ binds RGS14 at distinct sites to inhibit the RGS14:Gαi-AlF4- signaling complex and RGS14 nuclear localization. Authors - Kyle J Gerber View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RGS14 Polyclonal antibody

RGS14 Polyclonal Antibody for WB, IP, IHC, ELISA

Validation Data Gallery

WB analysis using 16258-1-AP

WB analysis of mouse brain using 16258-1-AP

WB analysis of mouse brain using 16258-1-AP

WB analysis of mouse spleen using 16258-1-AP

WB analysis of mouse testis using 16258-1-AP

IP experiment of mouse brain using 16258-1-AP

IHC staining of mouse brain using 16258-1-AP

IHC staining of mouse brain using 16258-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Hepatic Regulator of G protein Signaling 14 Ameliorates NAFLD through Activating cAMP-AMPK Signaling by Targeting Giα1/3

Regulator of G protein signaling 14 (RGS14) is expressed pre- and postsynaptically in neurons of hippocampus, basal ganglia, and amygdala of monkey and human brain.

Modelling cognitive deficits in Parkinson's disease: Is CA2 a gateway for hippocampal synucleinopathy?

Human genetic variants disrupt RGS14 nuclear shuttling and regulation of LTP in hippocampal neurons.

RGS14 regulates hormone-sensitive NPT2A-mediated renal phosphate uptake via binding to the NHERF1 scaffolding protein.

14-3-3γ binds RGS14 at distinct sites to inhibit the RGS14:Gαi-AlF4- signaling complex and RGS14 nuclear localization.