Validation Data Gallery
Filter:
Tested Applications
| Positive WB detected in | MCF-7 cells, PC-3 cells, SH-SY5Y cells, mouse brain tissue, mouse lung tissue, MDA-MB-231 cells, NCI-H1299 cells, SK-BR-3 cells |
| Positive IHC detected in | human testis tissue, human cerebellum tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 3 publications below |
| IHC | See 1 publications below |
| IF | See 1 publications below |
Product Information
12549-1-AP targets RGS17 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | RGS17 fusion protein Ag3399 相同性解析による交差性が予測される生物種 |
| Full Name | regulator of G-protein signaling 17 |
| Calculated molecular weight | 210 aa, 24 kDa |
| Observed molecular weight | 27 kDa |
| GenBank accession number | BC013117 |
| Gene Symbol | RGS17 |
| Gene ID (NCBI) | 26575 |
| RRID | AB_2179954 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UGC6 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for RGS17 antibody 12549-1-AP | Download protocol |
| IHC protocol for RGS17 antibody 12549-1-AP | Download protocol |
| IF protocol for RGS17 antibody 12549-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Signal Altered expression and function of regulator of G-protein signaling-17 (RGS17) in hepatocellular carcinoma. | ||
Sci Rep Regulator of G protein signaling 17 represents a novel target for treating cisplatin induced hearing loss. | ||