Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse brain tissue, SH-SY5Y cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
Product Information
17970-1-AP targets RGS9 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | RGS9 fusion protein Ag11976 相同性解析による交差性が予測される生物種 |
| Full Name | regulator of G-protein signaling 9 |
| Calculated molecular weight | 622 aa, 71 kDa |
| Observed molecular weight | 58 kDa, 70-75 kDa |
| GenBank accession number | BC022504 |
| Gene Symbol | RGS9 |
| Gene ID (NCBI) | 8787 |
| RRID | AB_2178508 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O75916 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
RGS9 is a member of the RGS family of signaling proteins that suppress the activity of G proteins by promoting their deactivation. There are two splice isoforms of RGS9 with quite different properties and patterns of expression. RGS9-1 is mainly found in the eye and is involved in regulation of phototransduction in rod and cone cells of the retina, while RGS9-2 is is expressed specifically in the striatum, a brain region involved in controlling movement, motivation, mood and addition. This antibody got about 58kD in the mouse eye and 70kD in the mouse brain in western blotting, both in line with their expected masses. Defects in RGS9 are a cause of prolonged electroretinal response suppression (PERRS).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for RGS9 antibody 17970-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Genes (Basel) Extended Phenotyping and Functional Validation Facilitate Diagnosis of a Complex Patient Harboring Genetic Variants in MCCC1 and GNB5 Causing Overlapping Phenotypes. |