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KD/KO Validated

RHCG Polyclonal antibody

Name: RHCG Polyclonal antibody
Brand: Proteintech
SKU: 11949-1-AP

RHCG Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 11949-1-AP

Synonyms

Tumor-related protein DRC2, RHGK, PDRC2, CDRC2, C15orf6

Validation Data Gallery

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AllWBIHC

WB analysis of HEK-293 using 11949-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 11949-1-AP (RHCG antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of human kidney using 11949-1-AP
human kidney tissue were subjected to SDS PAGE followed by western blot with 11949-1-AP (RHCG antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IHC staining of human pancreas cancer using 11949-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 11949-1-AP (RHCG antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human kidney using 11949-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 11949-1-AP (RHCG antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human kidney using 11949-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 11949-1-AP (RHCG antibody) at dilution of 1:200 (under 40x lens).
IHC staining of human kidney using 11949-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 11949-1-AP (RHCG antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human kidney using 11949-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 11949-1-AP (RHCG antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human kidney using 11949-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 11949-1-AP (RHCG antibody) at dilution of 1:25 (under 10x lens).
IHC staining of human kidney using 11949-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 11949-1-AP (RHCG antibody) at dilution of 1:25 (under 40x lens).

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Cat No. 11949-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HEK-293 cells, human kidney tissue
Positive IHC detected in	human kidney tissue, human pancreas cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 2 publications below
IHC	See 1 publications below
IF	See 1 publications below
CoIP	See 1 publications below

Product Information

11949-1-AP targets RHCG in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	RHCG fusion protein Ag2493 相同性解析による交差性が予測される生物種
Full Name	Rh family, C glycoprotein
Calculated molecular weight	479 aa, 53 kDa
Observed molecular weight	52-55 kDa
GenBank accession number	BC030965
Gene symbol	RHCG
Gene ID (NCBI)	51458
RRID	AB_2269482
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for RHCG antibody 11949-1-AP	Download protocol
IHC protocol for RHCG antibody 11949-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB,IF,CoIP	Kidney Int The ammonia transporter RhCG modulates urinary acidification by interacting with the vacuolar proton-ATPases in renal intercalated cells. Authors - Soline Bourgeois KO Validated View Article
human	WB,IHC	Aging (Albany NY) Rh type C-glycoprotein functions as a novel tumor suppressor gene by inhibiting tumorigenicity and metastasis in head and neck squamous cell carcinoma. Authors - Wenguang Xu View Article

製品

アプリケーション

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サポート

カスタム対応

プロモーション

企業情報

抗体

免疫アッセイ

サイトカイン・増殖因子

ChromoTek VHH（Nanobody®）試薬

サポートツール・試薬

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研究分野

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RHCG Polyclonal antibody

RHCG Polyclonal Antibody for WB, IHC, ELISA

Validation Data Gallery

WB analysis of HEK-293 using 11949-1-AP

WB analysis of human kidney using 11949-1-AP

IHC staining of human pancreas cancer using 11949-1-AP

IHC staining of human kidney using 11949-1-AP

IHC staining of human kidney using 11949-1-AP

IHC staining of human kidney using 11949-1-AP

IHC staining of human kidney using 11949-1-AP

IHC staining of human kidney using 11949-1-AP

IHC staining of human kidney using 11949-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

The ammonia transporter RhCG modulates urinary acidification by interacting with the vacuolar proton-ATPases in renal intercalated cells.

Rh type C-glycoprotein functions as a novel tumor suppressor gene by inhibiting tumorigenicity and metastasis in head and neck squamous cell carcinoma.