Validation Data Gallery

Filter: AllWBIHCIF-PIF/ICC

WB analysis using 15924-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 15924-1-AP (RHEB antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of fetal human brain using 15924-1-AP
fetal human brain tissue were subjected to SDS PAGE followed by western blot with 15924-1-AP (RHEB Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 15924-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 15924-1-AP (RHEB Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.
WB analysis of 3T3-L1 using 15924-1-AP
3T3-L1 cells were subjected to SDS PAGE followed by western blot with 15924-1-AP (RHEB Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of DU 145 using 15924-1-AP
DU 145 cells were subjected to SDS PAGE followed by western blot with 15924-1-AP (RHEB antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 15924-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 15924-1-AP (RHEB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IHC staining of human brain using 15924-1-AP
Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 15924-1-AP (RHEB antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human brain using 15924-1-AP
Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 15924-1-AP (RHEB antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 15924-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15924-1-AP (RHEB Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of mouse brain using 15924-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15924-1-AP (RHEB Antibody) at dilution of 1:50 (under 40x lens).
IF Staining of human pancreas cancer using 15924-1-AP
Immunofluorescent analysis of (4% PFA) fixed human pancreas cancer tissue using RHEB antibody (15924-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).
IF Staining of human pancreas cancer using 15924-1-AP
Immunofluorescent analysis of (4% PFA) fixed human pancreas cancer tissue using RHEB antibody (15924-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).
IF Staining of mouse pancreas using 15924-1-AP
Immunofluorescent analysis of (4% PFA) fixed mouse pancreas tissue using RHEB antibody (15924-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).
IF Staining of HeLa using 15924-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using RHEB antibody (15924-1-AP) at dilution of 1:100 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

Featured Product
KD/KO Validated

RHEB Polyclonal antibody

RHEB Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Cat No. 15924-1-AP

Publications(14)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Synonyms

RHEB2, Ras homolog enriched in brain, GTP-binding protein Rheb, GTP binding protein Rheb, EC:3.6.5.-

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (14)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	DU 145 cells, human brain tissue, HeLa cells, 3T3-L1 cells, fetal human brain tissue
Positive IHC detected in	human brain tissue, mouse brain tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	human pancreas cancer tissue
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)-P	IF-P : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 2 publications below
WB	See 8 publications below
IHC	See 4 publications below
IF	See 3 publications below

Product Information

15924-1-AP targets RHEB in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	RHEB fusion protein Ag8747 相同性解析による交差性が予測される生物種
Full Name	Ras homolog enriched in brain
Calculated molecular weight	184 aa, 20 kDa
Observed molecular weight	15-20 kDa
GenBank accession number	BC016155
Gene Symbol	RHEB
Gene ID (NCBI)	6009
RRID	AB_2285084
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q15382
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Rheb is a member of the Ras superfamily small GTPases that plays important roles in the regulation of protein synthesis and growth in response to nutrient and growth factors. It is well known to bind and activate mTOR, a serine/threonine kinase which augments protein synthesis. Rheb has GTPase activity and shuttles between a GDP-bound form and a GTP-bound form, and farnesylation of the protein is required for this activity.

Protocols

Product Specific Protocols
WB protocol for RHEB antibody 15924-1-AP	Download protocol
IHC protocol for RHEB antibody 15924-1-AP	Download protocol
IF protocol for RHEB antibody 15924-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
rat	WB	Cell Metab Crosstalk between glucocorticoid receptor and nutritional sensor mTOR in skeletal muscle. Authors - Noriaki Shimizu View Article
mouse	IHC	PLoS Biol RHEB/mTOR hyperactivity causes cortical malformations and epileptic seizures through increased axonal connectivity. Authors - Martina Proietti Onori View Article
mouse	IF	Metabolism Rheb1 promotes glucose-stimulated insulin secretion in human and mouse β-cells by upregulating GLUT expression. Authors - Yan Yang KO Validated View Article
mouse	IF	iScience Whitening of brown adipose tissue inhibits osteogenic differentiation via secretion of S100A8/A9 Authors - Ting Wang View Article
human		Front Cell Dev Biol PPT1 Reduction Contributes to Erianin-Induced Growth Inhibition in Oral Squamous Carcinoma Cells. Authors - Qingqiong Luo View Article
mouse	WB	Stem Cell Res Ther Chaperone-mediated autophagy plays an important role in regulating retinal progenitor cell homeostasis. Authors - Caixia Jin View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 15924-1-AP

WB analysis of fetal human brain using 15924-1-AP

WB analysis of HeLa using 15924-1-AP

WB analysis of 3T3-L1 using 15924-1-AP

WB analysis of DU 145 using 15924-1-AP

WB analysis of human brain using 15924-1-AP

IHC staining of human brain using 15924-1-AP

IHC staining of human brain using 15924-1-AP

IHC staining of mouse brain using 15924-1-AP

IHC staining of mouse brain using 15924-1-AP

IF Staining of human pancreas cancer using 15924-1-AP

IF Staining of human pancreas cancer using 15924-1-AP

IF Staining of mouse pancreas using 15924-1-AP

IF Staining of HeLa using 15924-1-AP

RHEB Polyclonal antibody

RHEB Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Crosstalk between glucocorticoid receptor and nutritional sensor mTOR in skeletal muscle.

RHEB/mTOR hyperactivity causes cortical malformations and epileptic seizures through increased axonal connectivity.

Rheb1 promotes glucose-stimulated insulin secretion in human and mouse β-cells by upregulating GLUT expression.

Whitening of brown adipose tissue inhibits osteogenic differentiation via secretion of S100A8/A9

PPT1 Reduction Contributes to Erianin-Induced Growth Inhibition in Oral Squamous Carcinoma Cells.

Chaperone-mediated autophagy plays an important role in regulating retinal progenitor cell homeostasis.