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RHEB Polyclonal antibody
RHEB Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (1)
Applications
WB, IHC, IF/ICC, IF-P, ELISA
Conjugate
Unconjugated
Cat no : 15924-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | DU 145 cells, human brain tissue, HeLa cells, 3T3-L1 cells, fetal human brain tissue |
| Positive IHC detected in | human brain tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human pancreas cancer tissue |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 8 publications below |
| IHC | See 3 publications below |
| IF | See 2 publications below |
Product Information
15924-1-AP targets RHEB in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | RHEB fusion protein Ag8747 相同性解析による交差性が予測される生物種 |
| Full Name | Ras homolog enriched in brain |
| Calculated molecular weight | 184 aa, 20 kDa |
| Observed molecular weight | 15-20 kDa |
| GenBank accession number | BC016155 |
| Gene symbol | RHEB |
| Gene ID (NCBI) | 6009 |
| RRID | AB_2285084 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Rheb is a member of the Ras superfamily small GTPases that plays important roles in the regulation of protein synthesis and growth in response to nutrient and growth factors. It is well known to bind and activate mTOR, a serine/threonine kinase which augments protein synthesis. Rheb has GTPase activity and shuttles between a GDP-bound form and a GTP-bound form, and farnesylation of the protein is required for this activity.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for RHEB antibody 15924-1-AP | Download protocol |
| IHC protocol for RHEB antibody 15924-1-AP | Download protocol |
| IF protocol for RHEB antibody 15924-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Metab Crosstalk between glucocorticoid receptor and nutritional sensor mTOR in skeletal muscle. | ||
PLoS Biol RHEB/mTOR hyperactivity causes cortical malformations and epileptic seizures through increased axonal connectivity. | ||
Metabolism Rheb1 promotes glucose-stimulated insulin secretion in human and mouse β-cells by upregulating GLUT expression.
| ||
iScience Whitening of brown adipose tissue inhibits osteogenic differentiation via secretion of S100A8/A9 | ||
Front Cell Dev Biol PPT1 Reduction Contributes to Erianin-Induced Growth Inhibition in Oral Squamous Carcinoma Cells. | ||
Stem Cell Res Ther Chaperone-mediated autophagy plays an important role in regulating retinal progenitor cell homeostasis. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Tanusree (Verified Customer) (12-18-2019) | Product worked well in WB at 1:500 dilution
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