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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RHOA Polyclonal antibody, PBS Only

RHOA Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 27213-1-PBS

Synonyms

ARHA, ARH12, RHO12, Rho cDNA clone 12, h12

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse kidney tissue using RHOA Polyclonal antibody (27213-1-AP)

    WB analysis of mouse kidney using 27213-1-AP (same clone as 27213-1-PBS)

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 27213-1-AP (RHOA antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27213-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach tissue using RHOA Polyclonal antibody (27213-1-AP)

    IHC staining of human stomach using 27213-1-AP (same clone as 27213-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 27213-1-AP (RHOA antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27213-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

27213-1-PBS targets RHOA in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RHOA fusion protein Ag25873 相同性解析による交差性が予測される生物種
Full Name ras homolog gene family, member A
Calculated molecular weight 22 kDa
Observed molecular weight 22 kDa
GenBank accession numberBC005976
Gene symbol RHOA
Gene ID (NCBI) 387
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

RhoA is a member of the Rho family of small GTPases, which cycle between inactive GDP-bound and active GTP-bound states and function as molecular switches in signal transduction cascades. Rho proteins promote reorganization of the actin cytoskeleton and regulate cell shape, attachment, and motility. Overexpression of RhoA is associated with tumor cell proliferation and metastasis. RhoA signalling is critical to many cellular processes including migration, mechanotransduction, and is often disrupted in carcinogenesis.