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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RHOA Recombinant antibody

RHOA Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

240880E5

Cat no : 82760-2-RR

Synonyms

ARHA, ARH12, RHO12, Rho cDNA clone 12, h12

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RHOA Recombinant antibody (82760-2-RR)

    WB analysis using 82760-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82760-2-RR (RHOA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using RHOA Recombinant antibody (82760-2-RR)

    IF Staining of HeLa using 82760-2-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using RHOA antibody (82760-2-RR, Clone: 240880E5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 82760-2-RR

    Affinity and Kinetic Characterization of 82760-2-RR

    Biolayer interferometry (BLl) kinetic assays of 82760-2-RR against Human RHOA were performed. The affinity constant is 1.18 nM.

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Tested Applications

Positive WB detected inHUVEC cells, HeLa cells, HL-60 cells, NIH/3T3 cells, mouse brain tissue, rat brain tissue
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82760-2-RR targets RHOA in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen RHOA fusion protein Ag1141 相同性解析による交差性が予測される生物種
Full Name ras homolog gene family, member A
Calculated molecular weight 22 kDa
Observed molecular weight 22 kDa
GenBank accession numberBC005976
Gene symbol RHOA
Gene ID (NCBI) 387
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RhoA is a member of the Rho family of small GTPases, which cycle between inactive GDP-bound and active GTP-bound states and function as molecular switches in signal transduction cascades. Rho proteins promote reorganization of the actin cytoskeleton and regulate cell shape, attachment, and motility. Overexpression of RhoA is associated with tumor cell proliferation and metastasis. RhoA signalling is critical to many cellular processes including migration, mechanotransduction, and is often disrupted in carcinogenesis.

Protocols

Product Specific Protocols
WB protocol for RHOA antibody 82760-2-RRDownload protocol
IF protocol for RHOA antibody 82760-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols