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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RHOBTB3 Polyclonal antibody

RHOBTB3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Cat no : 13945-1-AP

Synonyms

KIAA0878, RHOBTB3

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using RHOBTB3 Polyclonal antibody (13945-1-AP)

    WB analysis using 13945-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13945-1-AP (RHOBTB3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using RHOBTB3 Polyclonal antibody (13945-1-AP)

    IHC staining of mouse heart using 13945-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 13945-1-AP (RHOBTB3 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat adrenal gland tissue using RHOBTB3 Polyclonal antibody (13945-1-AP)

    IHC staining of rat adrenal gland using 13945-1-AP

    Immunohistochemical analysis of paraffin-embedded rat adrenal gland tissue slide using 13945-1-AP (RHOBTB3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using RHOBTB3 Polyclonal antibody (13945-1-AP)

    IHC staining of human placenta using 13945-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 13945-1-AP (RHOBTB3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse heart tissue using RHOBTB3 Polyclonal antibody (13945-1-AP)

    IHC staining of mouse heart using 13945-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 13945-1-AP (RHOBTB3 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inHEK-293T cells, HeLa cells, K-562 cells
Positive IHC detected inmouse heart tissue, human placenta tissue, rat adrenal gland tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13945-1-AP targets RHOBTB3 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RHOBTB3 fusion protein Ag5025 相同性解析による交差性が予測される生物種
Full Name Rho-related BTB domain containing 3
Calculated molecular weight 611 aa, 69 kDa
Observed molecular weight 69 kDa
GenBank accession numberBC041337
Gene symbol RHOBTB3
Gene ID (NCBI) 22836
RRIDAB_2877992
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for RHOBTB3 antibody 13945-1-APDownload protocol
IHC protocol for RHOBTB3 antibody 13945-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Theranostics

Circular RNA circRHOBTB3 represses metastasis by regulating the HuR-mediated mRNA stability of PTBP1 in colorectal cancer.

Authors - Jiaxin Chen
View Article
humanWB,IP

Cells

RhoBTB3 Functions as a Novel Regulator of Autophagy by Suppressing AMBRA1 Stability

Authors - Kyungho Kim
View Article