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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RHOC Monoclonal antibody (67542-1-Ig)

    WB analysis using 67542-1-Ig (same clone as 67542-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67542-1-Ig (RHOC antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67542-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using RHOC Monoclonal antibody (67542-1-Ig)

    WB analysis using 67542-1-Ig (same clone as 67542-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67542-1-Ig (RHOC antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67542-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human small intestine tissue using RHOC Monoclonal antibody (67542-1-Ig)

    IHC staining of human small intestine using 67542-1-Ig (same clone as 67542-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 67542-1-Ig (RHOC antibody) at dilution of 1:800 (under 40x lens). This data was developed using the same antibody clone with 67542-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using RHOC Monoclonal antibody (67542-1-Ig)

    IF Staining of HeLa using 67542-1-Ig (same clone as 67542-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RHOC antibody (67542-1-Ig, Clone: 1G1C1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67542-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of Jurkat cells using RHOC Monoclonal antibody (67542-1-Ig)

    FC experiment of Jurkat using 67542-1-Ig (same clone as 67542-1-PBS)

    1x10^6 Jurkat cells were intracellularly stained with 0.4 ug RHOC Monoclonal antibody (67542-1-Ig, Clone:1G1C1) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 67542-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HepG2 cells using RHOC Monoclonal antibody (67542-1-Ig)

    FC experiment of HepG2 using 67542-1-Ig (same clone as 67542-1-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.4 ug RHOC Monoclonal antibody (67542-1-Ig, Clone:1G1C1) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 67542-1-PBS in a different storage buffer formulation.

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RHOC Monoclonal antibody, PBS Only

RHOC Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 67542-1-PBS
Clone No.1G1C1

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

ARHC, ARH9, Rho-related GTP-binding protein RhoC, Rho cDNA clone 9, H9

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67542-1-PBS targets RHOC in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen RHOC fusion protein Ag14633 相同性解析による交差性が予測される生物種
Full Name ras homolog gene family, member C
Calculated molecular weight 22 kDa
Observed molecular weight 22 kDa
GenBank accession numberBC007245
Gene Symbol RHOC
Gene ID (NCBI) 389
RRIDAB_2882761
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP08134
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Ras homolog family member C (RHOC) encodes a Rho-related GTP-binding protein, which belongs to small GTPase superfamily and also Rho family. RHOC. Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. Expression of RHOC contributes to metastatic phenotype of melanoma cells and is associated with multiple cancers including gastric carcinomas, ovarian carcinomas and breast cancer as well.