Validation Data Gallery

Filter: AllWBIPIHC

WB analysis using 12290-1-AP
Various cells were subjected to SDS PAGE followed by western blot with 12290-1-AP (RHOF antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis using 12290-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 12290-1-AP (RHOF antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 12290-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 12290-1-AP (RHOF antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of COLO 320 using 12290-1-AP
COLO 320 cells were subjected to SDS PAGE followed by western blot with 12290-1-AP (RHOF antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IP experiment of COLO 320 using 12290-1-AP
IP result of anti-RHOF (IP:12290-1-AP, 4ug; Detection:12290-1-AP 1:300) with COLO 320 cells lysate 2400 ug.
IHC staining of human colon cancer using 12290-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer using 12290-1-AP (RHOF antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human colon cancer using 12290-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer using 12290-1-AP (RHOF antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human small intestine using 12290-1-AP
Immunohistochemical analysis of paraffin-embedded human small intestine using 12290-1-AP (RHOF antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human small intestine using 12290-1-AP
Immunohistochemical analysis of paraffin-embedded human small intestine using 12290-1-AP (RHOF antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human small intestine using 12290-1-AP
Immunohistochemical analysis of paraffin-embedded human small intestine using 12290-1-AP (RHOF antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human small intestine using 12290-1-AP
Immunohistochemical analysis of paraffin-embedded human small intestine using 12290-1-AP (RHOF antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human colon cancer using 12290-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer using 12290-1-AP (RHOF antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human colon cancer using 12290-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer using 12290-1-AP (RHOF antibody) at dilution of 1:50 (under 40x lens).

RHOF Polyclonal antibody

RHOF Polyclonal Antibody for WB, IP, IHC, ELISA

Cat No. 12290-1-AP

Publications(4)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Synonyms

ARHF, Rho family GTPase Rif, Rho in filopodia, RHOF, RIF

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (4)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	A549 cells, HeLa cells, human brain tissue, COLO 320 cells, mouse brain tissue, rat brain tissue
Positive IP detected in	COLO 320 cells
Positive IHC detected in	human colon cancer tissue, human small intestine tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 3 publications below
IHC	See 2 publications below
IF	See 1 publications below

Product Information

12290-1-AP targets RHOF in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	RHOF fusion protein Ag2943 相同性解析による交差性が予測される生物種
Full Name	ras homolog gene family, member F (in filopodia)
Calculated molecular weight	211 aa, 24 kDa
Observed molecular weight	24 kDa
GenBank accession number	BC018208
Gene Symbol	RHOF
Gene ID (NCBI)	54509
RRID	AB_10644126
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9HBH0
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for RHOF antibody 12290-1-AP	Download protocol
IHC protocol for RHOF antibody 12290-1-AP	Download protocol
IP protocol for RHOF antibody 12290-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	J Biol Chem Rho family small GTPase Rif regulates Wnt5a-Ror1-Dvl2 signaling and promotes lung adenocarcinoma progression Authors - Michiru Nishita View Article
human	WB	Int J Mol Sci An Integrated Multi-Omics Analysis Identifying Immune Subtypes of Pancreatic Cancer Authors - Yongcheng Su View Article
human,mouse	WB,IHC,IF	iScience RHOF activation of AKT/β-catenin signaling pathway drives acute myeloid leukemia progression and chemotherapy resistance Authors - Xin Wen KD Validated View Article
human	IHC	Virchows Arch High expression of RHOF is an effective diagnostic marker and a potential prognostic indicator for primary mediastinal large B-cell lymphoma Authors - Xinyi Zhu View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 12290-1-AP

WB analysis using 12290-1-AP

WB analysis of human brain using 12290-1-AP

WB analysis of COLO 320 using 12290-1-AP

IP experiment of COLO 320 using 12290-1-AP

IHC staining of human colon cancer using 12290-1-AP

IHC staining of human colon cancer using 12290-1-AP

IHC staining of human small intestine using 12290-1-AP

IHC staining of human small intestine using 12290-1-AP

IHC staining of human small intestine using 12290-1-AP

IHC staining of human small intestine using 12290-1-AP

IHC staining of human colon cancer using 12290-1-AP

IHC staining of human colon cancer using 12290-1-AP

RHOF Polyclonal antibody

RHOF Polyclonal Antibody for WB, IP, IHC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Rho family small GTPase Rif regulates Wnt5a-Ror1-Dvl2 signaling and promotes lung adenocarcinoma progression

An Integrated Multi-Omics Analysis Identifying Immune Subtypes of Pancreatic Cancer

RHOF activation of AKT/β-catenin signaling pathway drives acute myeloid leukemia progression and chemotherapy resistance

High expression of RHOF is an effective diagnostic marker and a potential prognostic indicator for primary mediastinal large B-cell lymphoma