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RHOF Polyclonal antibody

RHOF Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 12290-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

ARHF, Rho family GTPase Rif, Rho in filopodia, RHOF, RIF

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -


ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive WB detected inA549 cells, HeLa cells, human brain tissue, COLO 320 cells, mouse brain tissue, rat brain tissue
Positive IP detected inCOLO 320 cells
Positive IHC detected inhuman colon cancer tissue, human small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12290-1-AP targets RHOF in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RHOF fusion protein Ag2943 相同性解析による交差性が予測される生物種
Full Name ras homolog gene family, member F (in filopodia)
Calculated molecular weight 211 aa, 24 kDa
Observed molecular weight24 kDa
GenBank accession numberBC018208
Gene Symbol RHOF
Gene ID (NCBI) 54509
RRIDAB_10644126
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9HBH0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for RHOF antibody 12290-1-APDownload protocol
IHC protocol for RHOF antibody 12290-1-APDownload protocol
IP protocol for RHOF antibody 12290-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Biol Chem

Rho family small GTPase Rif regulates Wnt5a-Ror1-Dvl2 signaling and promotes lung adenocarcinoma progression

Authors - Michiru Nishita
humanWB

Int J Mol Sci

An Integrated Multi-Omics Analysis Identifying Immune Subtypes of Pancreatic Cancer

Authors - Yongcheng Su
human,mouseWB,IHC,IF

iScience

RHOF activation of AKT/β-catenin signaling pathway drives acute myeloid leukemia progression and chemotherapy resistance

Authors - Xin Wen
  • KD Validated
humanIHC

Virchows Arch

High expression of RHOF is an effective diagnostic marker and a potential prognostic indicator for primary mediastinal large B-cell lymphoma

Authors - Xinyi Zhu
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