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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RHOG Polyclonal antibody

RHOG Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 21576-1-AP

Synonyms

ARHG, RHOG

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RHOG Polyclonal antibody (21576-1-AP)

    WB analysis using 21576-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21576-1-AP (RHOG antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using RHOG Polyclonal antibody (21576-1-AP)

    WB analysis of Jurkat using 21576-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 21576-1-AP (RHOG antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using RHOG Polyclonal antibody (21576-1-AP)

    WB analysis of HeLa using 21576-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 21576-1-AP (RHOG Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using RHOG Polyclonal antibody (21576-1-AP)

    WB analysis of MCF-7 using 21576-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 21576-1-AP (RHOG Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inJurkat cells, HeLa cells, MCF-7 cells, K-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 21576-1-AP is RHOG Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RHOG fusion protein Ag16118 相同性解析による交差性が予測される生物種
Full Name ras homolog gene family, member G (rho G)
Calculated molecular weight 191 aa, 21 kDa
Observed molecular weight 21 kDa
GenBank accession numberBC104178
Gene symbol RHOG
Gene ID (NCBI) 391
RRIDAB_2878887
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RHOG, also named as ARHG, belongs to the small GTPase superfamily and Rho family. It is required for the formation of membrane ruffles during macropinocytosis. And it is required for the formation of cup-like structures during trans-endothelial migration of leukocytes. In case of Salmonella enterica infection, it is activated by SopB and SGEF, which induces cytoskeleton rearrangements and promotes bacterial entry. This antibody is specific to RHOG.

Protocols

Product Specific Protocols
WB protocol for RHOG antibody 21576-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols